Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection

doi:10.1021/acs.analchem.2c01163

	Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection
	Li, Jingxian 1; Luo, Mengyu 1; Yang, Hongfen 5; Ma, Chao 1; Cai, Ren 1; Tan, Weihong 1,2,3,4
刊名	ANALYTICAL CHEMISTRY
	2022-04-26
卷号	94
ISSN号	0003-2700
DOI	10.1021/acs.analchem.2c01163
通讯作者	Cai, Ren(cairen@hnu.edu.cn)
英文摘要	Based on luminol-capped Pt-tipped Au bimetallic nanorods (NRs) (L-Au-Pt NRs) as the anode emitter and SnS2 quantum dots (QDs) hybrid Eu metal organic frameworks (MOFs) (SnS(2)QDs@Eu MOFs) as the cathode emitter, a dual-signal electrochemiluminescence (ECL) platform was designed for the ultrasensitive and highly selective detection of kanamycin (KAN). Using a dual-signal output mod; the ratiometric ECL aptasensor largely eliminates false-positives or false-negatives by self-calibration in the KAN assay process. To stimulate the resonance energy transform (RET) system, the KAN aptamer and complementary DNA are introduced for conjugation between the donor and acceptor. With the specific recognition of target KAN by its aptamer, L-Au-Pt NRs-apt partially peels off from the electrode surface. Eventually, the RET system is removed, leading to an increasing cathode signal and a decreasing anode signal. In view of this phenomenon, the ratiometric aptasensor can quantify KAN from 1 pM to 10 nM with a low detection limit of 0.32 pM. This dual-signal ECL aptasensor exhibits great practical potential in environmental monitoring and food safety.
资助项目	National Natural Science Foundation of China[22004032] ; Hunan Provincial Natural Science Foundation of China[2021JJ20020]
WOS关键词	BIOSENSOR
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000792310600041
资助机构	National Natural Science Foundation of China ; Hunan Provincial Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130843]
专题	中国科学院合肥物质科学研究院
通讯作者	Cai, Ren
作者单位	1.Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn,Mol Sci & Biomed Lab, Coll Biol,State Key Lab Chemo Biosensing & Chemom, Changsha 410082, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Zhejiang Canc Hosp, Canc Hosp,Univ Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China 5.Univ Texas Austin, Austin, TX 78712 USA
推荐引用方式 GB/T 7714	Li, Jingxian,Luo, Mengyu,Yang, Hongfen,et al. Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection[J]. ANALYTICAL CHEMISTRY,2022,94.
APA	Li, Jingxian,Luo, Mengyu,Yang, Hongfen,Ma, Chao,Cai, Ren,&Tan, Weihong.(2022).Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection.ANALYTICAL CHEMISTRY,94.
MLA	Li, Jingxian,et al."Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection".ANALYTICAL CHEMISTRY 94(2022).