Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus

doi:10.1016/j.celrep.2022.111086

	Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus
	Gao, Chao 2; Gu, Jinge 5; Zhang, Hong 2; Jiang, Kai 2; Tang, Linlin 2; Liu, Ren 1; Zhang, Li 1; Zhang, Pengfei 1; Liu, Cong 3,4,5; Dai, Bin 2
刊名	CELL REPORTS
	2022-07-19
卷号	40
ISSN号	2211-1247
DOI	10.1016/j.celrep.2022.111086
通讯作者	Liu, Cong(liulab@sioc.ac.cn) ; Dai, Bin(daibin@sjtu.edu.cn) ; Song, Jie(sjie@sjtu.edu.cn)
英文摘要	Hyperosmotic stress as physiologic dysfunction can reduce the cell volume and then redistribute both protein concentration and ionic strength, but its effect on liquid-liquid phase separation (LLPS) is not well un-derstood. Here, we map the hyperosmotic-stress-induced nuclear LLPS of amyotrophic lateral sclerosis (ALS)-related proteins (fused in sarcoma [FUS], TAR DNA-binding protein 43 [TDP-43]). The dynamic and reversibility of FUS granules are continuable with the increase of hypertonic stimulation time, but those of TDP-43 granules decrease significantly. Strikingly, FUS granules, but not TDP-43 granules, contain essential chaperone Hsp40, which can protect amyloid protein from solid aggregation. Moreover, FUS nuclear gran-ules can co-localize with paraspeckles, but not promyelocytic leukemia (PML) bodies or nuclear speckles, while TDP-43 nuclear granules cannot co-localize with the above nuclear bodies. Together, these results may broaden our understanding of the LLPS of ALS-related proteins in response to cellular stress.
资助项目	National Natural Science Foundation of China[82188101] ; National Natural Science Foundation of China[22161132008] ; National Key R&D Program of China[2021YFF1200200] ; Natural Science Foundation of Shanghai, China[19520714100] ; Natural Science Foundation of Shanghai, China[19ZR1475800] ; Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study[SNZJU-SIAS-006] ; Science and Technology Commission of Shanghai Municipality (STCSM)[20XD1425000] ; Science and Technology Commission of Shanghai Municipality (STCSM)[2019SHZDZX02] ; CAS project for Young Scientists in Basic Research[YSBR-009] ; Shanghai Pilot Program for Basic Research - Chinese Anatomical Society, Shanghai Branch[CYJ-SHFY-2022-005] ; Shanghai Pujiang Program[19PJ1405000]
WOS关键词	DNA-BINDING PROTEIN ; GRANULES ; TDP-43 ; GENE ; FUS ; TRANSITION ; TELOMERES ; CHAPERONE ; PROMOTES ; DROPLETS
WOS研究方向	Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000883795600002
资助机构	National Natural Science Foundation of China ; National Key R&D Program of China ; Natural Science Foundation of Shanghai, China ; Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study ; Science and Technology Commission of Shanghai Municipality (STCSM) ; CAS project for Young Scientists in Basic Research ; Shanghai Pilot Program for Basic Research - Chinese Anatomical Society, Shanghai Branch ; Shanghai Pujiang Program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130421]
专题	中国科学院合肥物质科学研究院
通讯作者	Liu, Cong; Dai, Bin; Song, Jie
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Canc Hosp, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Inst Nano Biomed & Engn, Dept Instrument Sci & Engn, Shanghai 200240, Peoples R China 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Inst Neurol, Sch Med, Shanghai 200025, Peoples R China 4.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Neurol, Shanghai 200025, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Organ Chem, Interdisciplinary Res Ctr Biol & Chem, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Gao, Chao,Gu, Jinge,Zhang, Hong,et al. Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus[J]. CELL REPORTS,2022,40.
APA	Gao, Chao.,Gu, Jinge.,Zhang, Hong.,Jiang, Kai.,Tang, Linlin.,...&Song, Jie.(2022).Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.CELL REPORTS,40.
MLA	Gao, Chao,et al."Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus".CELL REPORTS 40(2022).