Effects of Sensitized Sorafenib with a Paeoniflorin and Geniposide Mixture on Liver Cancer via the NF-kappa B-HIF-2 alpha-SerpinB3 Pathway

doi:10.1155/2022/1911311

	Effects of Sensitized Sorafenib with a Paeoniflorin and Geniposide Mixture on Liver Cancer via the NF-kappa B-HIF-2 alpha-SerpinB3 Pathway
	Li, Jun-Fei; Zheng, Xiao-Rong; Zhang, Hong-Yan; Shen, Chang-Ming; Shen, Guo-xin; Jiang, Jian-Wei
刊名	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
	2022-10-15
卷号	2022
ISSN号	1741-427X
DOI	10.1155/2022/1911311
通讯作者	Jiang, Jian-Wei(jiangjw@zjcc.org.cn)
英文摘要	Purpose. This study focused on determining the anticancer effect of paeoniflorin and geniposide mixture (PFGS) combined with sorafenib (Sor) in hepatocellular carcinoma (HCC) and, in particular, whether PFGS increases the antitumor effect of Sor by modulating the NF-kappa B/HIF-2 alpha/SerpinB3 pathway. Methods. The H22 hepatoma tumor-bearing mouse model was treated with PFGS, Sor, and a combination of the two drugs for 12 days. The effects of PFGS combined with Sor on tumor growth and apoptosis and the expression of NF-kappa B, HIF-2 alpha, and SerpinB3 in tumor tissue were assessed. In addition, Sor-resistant hepatoma cells were treated with PFGS, Sor, and the combination of the two drugs in vitro. The effects of PFGS combined with Sor on cell proliferation and invasion and the protein expression of NF-kappa B p65, HIF-2 alpha, and SerpinB3 were investigated. Results. PFGS combined with Sor treatment synergistically inhibited tumor growth in HCC tumor-bearing mice. Immunostaining showed that PFGS combined with Sor treatment significantly decreased the expression of Ki-67 and obviously induced apoptosis in the tumor compared with a single treatment. Similarly, PFGS combined with Sor treatment significantly downregulated the expression of NF-kappa B, HIF-2 alpha, and SerpinB3 in the tumor compared with a single treatment. Additionally, PFGS combined with Sor markedly inhibited cell proliferation and invasion and activation of the NF-kappa B/HIF-2 alpha/SerpinB3 pathway in Sor-resistant hepatoma cells compared with a single treatment. Conclusion. Our study demonstrated that PFGS synergistically increased the antiliver cancer effects of Sor by lowering activation of the NF-kappa B/HIF-2 alpha/SerpinB3 pathway. These findings provided a scientific foundation for clinical studies using PFGS and Sor to treat liver cancer.
资助项目	Zhejiang Province Medical Health Science and Technology Project[2020KY074] ; Zhejiang provincial Science and Technology Plan of Traditional Chinese Medicine[2022ZA025]
WOS关键词	NF-KAPPA-B ; HEPATOCELLULAR-CARCINOMA ; EXPRESSION ; RESISTANCE ; SERPINB3 ; METASTASIS ; SYNERGIZES ; AUTOPHAGY ; EFFICACY
WOS研究方向	Integrative & Complementary Medicine
语种	英语
出版者	HINDAWI LTD
WOS记录号	WOS:000876508200001
资助机构	Zhejiang Province Medical Health Science and Technology Project ; Zhejiang provincial Science and Technology Plan of Traditional Chinese Medicine
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130041]
专题	中国科学院合肥物质科学研究院
通讯作者	Jiang, Jian-Wei
作者单位	Univ Chinese Acad Sci, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC, Chinese Acad Sci,Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Li, Jun-Fei,Zheng, Xiao-Rong,Zhang, Hong-Yan,et al. Effects of Sensitized Sorafenib with a Paeoniflorin and Geniposide Mixture on Liver Cancer via the NF-kappa B-HIF-2 alpha-SerpinB3 Pathway[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2022,2022.
APA	Li, Jun-Fei,Zheng, Xiao-Rong,Zhang, Hong-Yan,Shen, Chang-Ming,Shen, Guo-xin,&Jiang, Jian-Wei.(2022).Effects of Sensitized Sorafenib with a Paeoniflorin and Geniposide Mixture on Liver Cancer via the NF-kappa B-HIF-2 alpha-SerpinB3 Pathway.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2022.
MLA	Li, Jun-Fei,et al."Effects of Sensitized Sorafenib with a Paeoniflorin and Geniposide Mixture on Liver Cancer via the NF-kappa B-HIF-2 alpha-SerpinB3 Pathway".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022(2022).