CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice

doi:10.1186/s13068-022-02205-y

	CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice
	Jiang, Hongrui 1,2; Ren, Yan 1,2; Guo, Junyao 1,2; Yang, Huijie 1,2; Zhu, Xiaotong 1,2; Li, Wenhao 1,2; Tao, Liangzhi 1,2; Zhan, Yue 1,2; Wang, Qi 1,2; Wu, Yuejin 1,2
刊名	BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
	2022-10-14
卷号	15
关键词	Secondary cell wall (SCW) Rice Cellulose biosynthesis Biomass Saccharification membrane trafficking Map-based cloning
DOI	10.1186/s13068-022-02205-y
通讯作者	Liu, Binmei(liubm@ipp.ac.cn) ; Ye, Yafeng(yyfeng86@163.com)
英文摘要	Background As one of the most important staple food crops, rice produces large of agronomic biomass residues that contain lots of secondary cell walls (SCWs). Membrane trafficking plays key roles in SCWs biosynthesis, but information association membrane trafficking and SCWs formation in plants is limited. Results In this study, we report the function characterization of a rice mutant, culm easily fragile 3 (cef3), that exhibits growth retardation and fragile culm phenotype with significantly altered cell wall composition and reduced secondary wall thickness. Map-based cloning revealed that CEF3 encodes a homologous protein of Arabidopsis STOMATAL CYTOKINESIS DEFECTIVE2 (SCD2). The saccharification assays revealed that CEF3 mutation can improve biomass enzymatic saccharification. Expression pattern analysis indicated that CEF3 is ubiquitously expressed in many organs at different developmental stages. Subcellular localization revealed that CEF3 is a Golgi-localized protein. The FM4-64 uptake assay revealed CEF3 is involved in endocytosis. Furthermore, mutation of CEF3 not only affected cellulose synthesis-related genes expression, but also altered the abundance of cellulose synthase catalytic subunit 9 (OsCESA9) in the PM and in the endomembrane systems. Conclusions This study has demonstrated that CEF3 participates in the membrane trafficking that is essential for normal cellulose and other polysaccharides biosynthesis of the secondary cell wall, thereby manipulation of CEF3 could alter cellulose content and enhance biomass enzymatic saccharification in rice plants. Therefore, the study of the function of CEF3 can provide a strategy for genetic modification of SCWs in bioenergy crops.
资助项目	Natural Science Foundation of Anhui Province[2108085MC99] ; National Natural Science Foundation of China[32171962] ; Youth Innovation Promotion Association CAS[2022454] ; State Key Laboratory of Plant Cell and Chromosome Engineering[PCCE-KF-2021-01] ; HFIPS Director's Fund[YZJJKX202201] ; Hefei Science and Technology Project[J2020G45]
WOS关键词	PLANT SECRETORY PATHWAY ; BIOFUEL PRODUCTION ; CELLULOSE ; PROTEIN ; ENCODES ; RESISTANCE ; COMPLEXES ; DRP2B ; LEADS
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BMC
WOS记录号	WOS:000873907700001
资助机构	Natural Science Foundation of Anhui Province ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; State Key Laboratory of Plant Cell and Chromosome Engineering ; HFIPS Director's Fund ; Hefei Science and Technology Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129783]
专题	中国科学院合肥物质科学研究院
通讯作者	Liu, Binmei; Ye, Yafeng
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Hongrui,Ren, Yan,Guo, Junyao,et al. CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice[J]. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,2022,15.
APA	Jiang, Hongrui.,Ren, Yan.,Guo, Junyao.,Yang, Huijie.,Zhu, Xiaotong.,...&Ye, Yafeng.(2022).CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice.BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,15.
MLA	Jiang, Hongrui,et al."CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice".BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 15(2022).