A novel fluorescent digitonin derivative for non-invasive skin cholesterol detection: potential application in atherosclerosis screening

doi:10.1039/d2ra01982e

	A novel fluorescent digitonin derivative for non-invasive skin cholesterol detection: potential application in atherosclerosis screening
	Ni, Jingshu 2,3; Liu, Yong 2,3,4; Hong, Haiou 5; Kong, Xiangyong 5; Han, Yongsheng 5; Lei Zhang 1; Zhang, Yang 2,3; Zhang, Yuanzhi 2,4; Hua, Changyi 2; Wang, Quanfu 2
刊名	RSC ADVANCES
	2022-06-22
卷号	12
DOI	10.1039/d2ra01982e
通讯作者	Wang YiKun(wyk@aiofm.ac.cn) ; Dong Meili(dongmeili@aiofm.ac.cn)
英文摘要	There is a great demand for the rapid and non-invasive atherosclerosis screening method. Cholesterol content in the epidermis of the skin is an early biomarker for atherosclerosis. Risk assessment of atherosclerosis can be achieved by measuring cholesterol in the epidermis. Here, we synthesised a new fluorescent digitonin derivative (FDD) for the non-invasive detection of skin cholesterol. The results of fluorescence spectroscopy studies indicated that the probe exhibited desirable selectivity for cholesterol. The proof-of-concept preclinical study confirmed that FDD can detect different concentrations of skin cholesterol; patients diagnosed with atherosclerotic cardiovascular disease and the at-risk atherosclerosis group exhibited higher skin cholesterol content than the normal group. The area under the ROC curve for distinguishing the normal/disease group was 0.9228 (95% confidence interval, 0.8938 to 0.9518), and the area under the ROC curve for distinguishing the normal/risk group was 0.9422 (95% confidence interval, 0.9178 to 0.9665). We anticipate that this non-invasive skin cholesterol test may be used as a risk assessment tool for atherosclerosis screening in a large population for further examination and intervention in high-risk populations.
资助项目	Science and Technology Major Project of Anhui Province of China[201903a07020027] ; Science and Technology Service Network Project, Chinese Academy of Sciences[KFJ-STS-QYZD-184] ; Natural Science Foundation of Anhui Province of China[1908085QH365] ; Key Research and Development Program of Anhui Province of China[202103a07020008] ; Key Research and Development Program of Anhui Province of China[202004a07020016] ; Key Research and Development Program of Anhui Province of China[202104a07020023] ; President's Foundation of Hefei Institute of Physical Science, Chinese Academy of Sciences[YZJJZX202009] ; First Affiliated Hospital of University of Science and Technology of China
WOS关键词	TISSUE CHOLESTEROL ; RISK ; PERMEABILITY
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000814225900001
资助机构	Science and Technology Major Project of Anhui Province of China ; Science and Technology Service Network Project, Chinese Academy of Sciences ; Natural Science Foundation of Anhui Province of China ; Key Research and Development Program of Anhui Province of China ; President's Foundation of Hefei Institute of Physical Science, Chinese Academy of Sciences ; First Affiliated Hospital of University of Science and Technology of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129602]
专题	中国科学院合肥物质科学研究院
通讯作者	Wang YiKun; Dong Meili
作者单位	1.Anhui Prov Inst Food & Drug Control, Hefei 230051, Peoples R China 2.Chinese Acad Sci, Anhui Prov Engn Technol Res Ctr Biomed Opt Instru, Anhui Inst Opt & Fine Mech, Hefei Inst Phys Sci,Anhui Prov Engn Lab Med Opt D, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China 4.Wanjiang Ctr Dev Emerging Ind Technol, Tongling 244000, Peoples R China 5.Univ Sci & Technol China, Affiliated Hosp USTC 1, Div Life Sci & Med, Hefei 230001, Peoples R China
推荐引用方式 GB/T 7714	Ni, Jingshu,Liu, Yong,Hong, Haiou,et al. A novel fluorescent digitonin derivative for non-invasive skin cholesterol detection: potential application in atherosclerosis screening[J]. RSC ADVANCES,2022,12.
APA	Ni, Jingshu.,Liu, Yong.,Hong, Haiou.,Kong, Xiangyong.,Han, Yongsheng.,...&Dong Meili.(2022).A novel fluorescent digitonin derivative for non-invasive skin cholesterol detection: potential application in atherosclerosis screening.RSC ADVANCES,12.
MLA	Ni, Jingshu,et al."A novel fluorescent digitonin derivative for non-invasive skin cholesterol detection: potential application in atherosclerosis screening".RSC ADVANCES 12(2022).