Integrated microbiome, metabolome, and proteome analysis identifies a novel interplay among commensal bacteria, metabolites and candidate targets in non-small cell lung cancer

doi:10.1002/ctm2.947

	Integrated microbiome, metabolome, and proteome analysis identifies a novel interplay among commensal bacteria, metabolites and candidate targets in non-small cell lung cancer
	Qian, Xiang 2; Zhang, Hong-Yan 1,2; Li, Qing-Lin 2; Ma, Guan-Jun 4; Chen, Zhuo 2; Ji, Xu-Ming 3; Li, Chang-Yu 3; Zhang, Ai-Qin 2
刊名	CLINICAL AND TRANSLATIONAL MEDICINE
	2022-06-01
卷号	12
关键词	all-trans-retinoic acid gut microbiota lung cancer metabonomics nervonic acid proteomics
ISSN号	2001-1326
DOI	10.1002/ctm2.947
通讯作者	Li, Chang-Yu(lm159@sina.com) ; Zhang, Ai-Qin(zhangaq@zjcc.org.cn)
英文摘要	Background Accumulation of evidence suggests that the gut microbiome, its specific metabolites, and differentially expressed proteins (DEPs) are related to non-small cell lung cancer (NSCLC) pathogenesis. We now report the influences of the gut microbiota, metabolites, and DEPs on the mediation of NSCLC's chronic inflammation and immune dysregulation. Methods We conducted 16S ribosomal RNA sequencing for the gut microbiome in healthy volunteers and NSCLC patients. Liquid chromatography-mass spectrometry (LC-MS) analysis was employed to explore differences between metabolites and DEPs in serum samples. Additionally, LC-MS-based metabolomic analysis was conducted in 40 NSCLC tissues and 40 adjacent tissues. The omics data were separately analysed and integrated by using Spearman's correlation coefficient. Then, faecal microbiota transplantation (FMT) assay was used to assess the effects of the gut microbiome and specific metabolites in mice. Results Faecal microbiome analysis revealed gut microflora dysbiosis in NSCLC patients with Prevotella, Gemmiger, and Roseburia significantly upregulated at the genus level. Then, we identified that nervonic acid/all-trans-retinoic acid level was negatively related to Prevotella. Additionally, a total of core 8 DEPs were selected in the proteome analysis, which mainly participated in the production of IL-8 and NF-kappa B pathways. CRP, LBP, and CD14 were identified as potential biomarkers for NSCLC. Transplantation of faecal microbiota from patients with NSCLC or Prevotella copri-colonized recipient in mice resulted in inflammation and immune dysregulation. In turn, nervonic acid/all-trans-retinoic acid treatment improved the phenotype of C57BL/6 mice bearing P. copri-treated Lewis lung cancer (LLC). Conclusions Overall, these results pointed out that P. copri-nervonic acid/all-trans-retinoic acid axis may contribute to the pathogenesis of NSCLC.
WOS关键词	GUT MICROBIOME ; BIOMARKERS ; DIAGNOSIS ; PREDICT
WOS研究方向	Oncology ; Research & Experimental Medicine
语种	英语
出版者	JOHN WILEY & SONS LTD
WOS记录号	WOS:000814529800001
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129588]
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Chang-Yu; Zhang, Ai-Qin
作者单位	1.Zhejiang Prov Key Lab Thorac Tumor, Hangzhou, Peoples R China 2.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, 1 East Banshan Rd, Hangzhou 310022, Peoples R China 3.Zhejiang Chinese Med Univ, 548 Binwen Rd, Hangzhou 310053, Zhejiang, Peoples R China 4.Zhejiang Univ, Affiliated Hangzhou Canc Hosp, Dept Comprehens Ward, Sch Med, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Qian, Xiang,Zhang, Hong-Yan,Li, Qing-Lin,et al. Integrated microbiome, metabolome, and proteome analysis identifies a novel interplay among commensal bacteria, metabolites and candidate targets in non-small cell lung cancer[J]. CLINICAL AND TRANSLATIONAL MEDICINE,2022,12.
APA	Qian, Xiang.,Zhang, Hong-Yan.,Li, Qing-Lin.,Ma, Guan-Jun.,Chen, Zhuo.,...&Zhang, Ai-Qin.(2022).Integrated microbiome, metabolome, and proteome analysis identifies a novel interplay among commensal bacteria, metabolites and candidate targets in non-small cell lung cancer.CLINICAL AND TRANSLATIONAL MEDICINE,12.
MLA	Qian, Xiang,et al."Integrated microbiome, metabolome, and proteome analysis identifies a novel interplay among commensal bacteria, metabolites and candidate targets in non-small cell lung cancer".CLINICAL AND TRANSLATIONAL MEDICINE 12(2022).