Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice

doi:10.3389/fpls.2022.997624

	Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice
	Ye, Yafeng 1,2; Wang, Shuoxun 3; Ren, Yan 1,2; Yang, Huijie 1,2; Guo, Junyao 1,2; Jiang, Hongrui 1,2; Zhu, Xiaotong 1,2; Li, Wenhao 1,2; Tao, Liangzhi 1,2; Zhan, Yue 1,2
刊名	FRONTIERS IN PLANT SCIENCE
	2022-09-13
卷号	13
关键词	grain size rice grain yield LGW GW7 gene expression
ISSN号	1664-462X
DOI	10.3389/fpls.2022.997624
通讯作者	Ye, Yafeng(yyfeng86@163.com) ; Wu, Kun(kunwu@genetics.ac.cn) ; Liu, Binmei(liubm@ipp.ac.cn)
英文摘要	Grain weight is a major determinant in rice yield, which is tightly associated with grain size. However, the underlying molecular mechanisms that control this trait remain unclear. Here, we report a rice (Oryza sativa) mutant, low grain weight (lgw), which shows that reduced grain length is caused by decreased cell elongation and proliferation. Map-based cloning revealed that all mutant phenotypes resulted from a nine-base pair (bp) deletion in LGW, which encodes the kinesin-like protein BRITTLE CULM12 (BC12). Protein sequence alignment analysis revealed that the mutation site was located at the nuclear localization signal (NLS) of LGW/BC12, resulting in the lgw protein not being located in the nucleus. LGW is preferentially expressed in both culms and roots, as well as in the early developing panicles. Overexpression of LGW increased the grain length, indicating that LGW is a positive regulator for regulating grain length. In addition, LGW/BC12 is directly bound to the promoter of GW7 and activates its expression. Elevating the GW7 expression levels in lgw plants rescued the small grain size phenotype. We conclude that LGW regulates grain development by directly binding to the GW7 promoter and activating its expression. Our findings revealed that LGW plays an important role in regulating grain size, and manipulation of this gene provides a new strategy for regulating grain weight in rice.
资助项目	National Natural Science Foundation of China[32171962] ; Youth Innovation Promotion Association CAS[2022454] ; Natural Science Foundation of Anhui Province[2108085MC99] ; State Key Laboratory of Plant Cell and Chromosome Engineering[PCCE-KF-2021-01] ; HFIPS Director's Fund[YZJJKX202201] ; Hefei Science and Technology Project[J2020G45] ; Heavy Ion Research Facility in Lanzhou[HIR2021PY014]
WOS关键词	ACTIVATED PROTEIN-KINASE ; NATURAL VARIATION ; ENCODES ; SHAPE ; GENE ; BIOSYNTHESIS ; PROMOTER ; DWARFISM ; YIELD ; WIDTH
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000861906700001
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Natural Science Foundation of Anhui Province ; State Key Laboratory of Plant Cell and Chromosome Engineering ; HFIPS Director's Fund ; Hefei Science and Technology Project ; Heavy Ion Research Facility in Lanzhou
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129181]
专题	中国科学院合肥物质科学研究院
通讯作者	Ye, Yafeng; Wu, Kun; Liu, Binmei
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Contr, Hefei, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ye, Yafeng,Wang, Shuoxun,Ren, Yan,et al. Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Ye, Yafeng.,Wang, Shuoxun.,Ren, Yan.,Yang, Huijie.,Guo, Junyao.,...&Liu, Binmei.(2022).Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice.FRONTIERS IN PLANT SCIENCE,13.
MLA	Ye, Yafeng,et al."Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice".FRONTIERS IN PLANT SCIENCE 13(2022).