Effect of BMP-Wnt-Nodal signal on stem cell differentiation

	Effect of BMP-Wnt-Nodal signal on stem cell differentiation
	Wang, LY; Ma, RH; Jia, GX; Jian, SY; Zeng, XH; Xiong, ZF; Li, BY; Li, C
刊名	ZYGOTE
	2022
卷号	30 期号:1 页码:138
英文摘要	The generation of germ cells from embryonic stem cells in vitro has current historical significance. Western blot, qPCR, immunofluorescence and flow cytometry assays were used to investigate the differences in expression levels of totipotency and specific markers for Wnt regulation and the related signalling pathways during primordial germ cell-like cell (PGCLC) induction and differentiation. During PGCLC induction, activation of WNT3a increased the expression of NANOG, SOX2 and OCT4, but Mvh, DAZL, Blimp1, TFAP2C, Gata4, SOX17, EOMES, Brachyury and PRDM1 expression levels were significantly reduced. Inhibition of the WNT signal demonstrated the opposite effect. Similarly, inhibitors of BMP and the Nodal/Activin signal were used to determine the effect of signal pathways on differentiation. CER1 affected the Wnt signal and differentiation, but the inhibitor SB only regulated differentiation. BMP-WNT-NODAL were mainly responsible for regulating differentiation. Our results provide a reliable theoretical basis and feasibility for further clinical medical research.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61302]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, LY,Ma, RH,Jia, GX,et al. Effect of BMP-Wnt-Nodal signal on stem cell differentiation[J]. ZYGOTE,2022,30(1):138.
APA	Wang, LY.,Ma, RH.,Jia, GX.,Jian, SY.,Zeng, XH.,...&Li, C.(2022).Effect of BMP-Wnt-Nodal signal on stem cell differentiation.ZYGOTE,30(1),138.
MLA	Wang, LY,et al."Effect of BMP-Wnt-Nodal signal on stem cell differentiation".ZYGOTE 30.1(2022):138.