Nature-inspired nanozymes as signal markers for in-situ signal amplification strategy: A portable dual-colorimetric immunochromatographic analysis based on smartphone

	Nature-inspired nanozymes as signal markers for in-situ signal amplification strategy: A portable dual-colorimetric immunochromatographic analysis based on smartphone
	Li, YC; Liu, SJ; Yin, XC; Wang, SC; Tian, YL; Shu, R; Jia, CH; Chen, YQ; Sun, J; Zhang, DH
刊名	BIOSENSORS & BIOELECTRONICS
	2022
卷号	210
英文摘要	Owing to the lack of a universal descriptor to predict the nanozymes as signal markers (SM) of immunochromatographic analysis (ICA), the present exploitation of nanozymes as SM heavily relies on trial-and-error strategies, which obstructs the rational design of nanozymes with monoclonal antibodies (mAbs) recognition activity. Herein, inspired by the structure of the active center of natural multi-iron peroxidases and polyphenolprotein interactions, a rational design of an artificial peroxidase with mAbs recognition activity by utilizing gallic acid (GA) to chelate with multivalent iron was successfully proposed by utilizing gallic acid (GA) to chelate with multivalent iron. The most essential features of peroxidase-like Fe-GA nanozymes (FGN) were investigated, showing high catalytic performance and good stability. Subsequently, FGN was employed as SM for mAbs in ICA, which played the following triple roles in the ICA sensor: (i) the direct recognizer of mAbs; (ii) the generator of original colorimetric signal; (iii) the generator of catalytic in-suit amplification colorimetric signal. To make the ICA more portable, we have employed a smartphone and principal component analysis (PCA) to assist this on-site detection. As a proof-of-concept, clenbuterol (CLL) was analyzed by the proposed nanozymes-mediated dualcolorimetric ICA based on a smartphone. Notably, the proposed dual-colorimetric ICA exhibits high analytical performance for the quantification of CLL in the detection range of 0-6 ng mL-1 with a limit of detection (LOD) as low as 0.172 ng mL-1. Meanwhile, the proposed dual-colorimetric ICA exhibits remarkable feasibility and was successfully employed for the detection of CLL in pork and chicken matrixes.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61134]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, YC,Liu, SJ,Yin, XC,et al. Nature-inspired nanozymes as signal markers for in-situ signal amplification strategy: A portable dual-colorimetric immunochromatographic analysis based on smartphone[J]. BIOSENSORS & BIOELECTRONICS,2022,210.
APA	Li, YC.,Liu, SJ.,Yin, XC.,Wang, SC.,Tian, YL.,...&Wang, JL.(2022).Nature-inspired nanozymes as signal markers for in-situ signal amplification strategy: A portable dual-colorimetric immunochromatographic analysis based on smartphone.BIOSENSORS & BIOELECTRONICS,210.
MLA	Li, YC,et al."Nature-inspired nanozymes as signal markers for in-situ signal amplification strategy: A portable dual-colorimetric immunochromatographic analysis based on smartphone".BIOSENSORS & BIOELECTRONICS 210(2022).