Engineered Core-Shell Multifunctional Nano-Tracer in Raman-Silent Region with Highly Retained Affinity to Enhance Lateral Flow Immunoassays

	Engineered Core-Shell Multifunctional Nano-Tracer in Raman-Silent Region with Highly Retained Affinity to Enhance Lateral Flow Immunoassays
	Liu, SJ; Luo, X; Shu, R; Liao, YJ; Dou, LN; Bu, T; Wang, SC; Li, YC; Sun, J; Zhang, DH
刊名	SMALL
	2022
卷号	18 期号:45
英文摘要	Stimulated surface-enhanced Raman scattering (SERS) in combination with engineered nano-tracer offers extraordinary potential in lateral flow immunoassays (LFIAs). Nonetheless, the investigation execution of SERS-LFIA is often compromised by the intricacy and overlap of the Raman fingerprint spectrum as well as the affinity-interference of nano-tracer to antibody. To circumvent these critical issues, an engineered core-shell multifunctional nano-tracer (named APNPs) with precise control of the size of nano-core (AuNPs) and coating of the nano-shell (Prussian blue nanomaterials) is prepared for SERS-LFIA via a modified enlarging particle size and coating modification strategy. Importantly, this nano-tracer exhibits enhanced coupling efficiency, highly retained affinity, reinforced colloid stability, and unique SERS signal (2156 cm(-1)) in the silent region (1800-2800 cm(-1)) with high signal-to-background ratio simultaneously, all of which are beneficial to the enhancement of the analysis performance. With a proof-of-concept demonstration for detection of ractopamine (RAC), a dual-pattern LFIA that synergizes both the enlarged particle size and coating modification supported colorimetric/biological silence Raman dual-response (coined as the ECCRD assay) is demonstrated by integrating APNPs with the competitive-type immunoreaction. This research may contribute to the rational design of multifunctional nano-tracer, and the ECCRD assay can be expanded for a wide spectrum of applications in environmental monitoring and biomedical diagnosis.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61102]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, SJ,Luo, X,Shu, R,et al. Engineered Core-Shell Multifunctional Nano-Tracer in Raman-Silent Region with Highly Retained Affinity to Enhance Lateral Flow Immunoassays[J]. SMALL,2022,18(45).
APA	Liu, SJ.,Luo, X.,Shu, R.,Liao, YJ.,Dou, LN.,...&Wang, JL.(2022).Engineered Core-Shell Multifunctional Nano-Tracer in Raman-Silent Region with Highly Retained Affinity to Enhance Lateral Flow Immunoassays.SMALL,18(45).
MLA	Liu, SJ,et al."Engineered Core-Shell Multifunctional Nano-Tracer in Raman-Silent Region with Highly Retained Affinity to Enhance Lateral Flow Immunoassays".SMALL 18.45(2022).