Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food

	Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food
	Li, YC; Zhang, H; Cui, ZW; Liu, SJ; Xu, JK; Jia, CH; Chen, YQ; Wang, LL; Sun, J; Zhang, DH
刊名	FOOD CHEMISTRY
	2023
卷号	400
英文摘要	Exploring a novel strategy for strengthening the catalytic activity of enzyme facilitates the development of a sensitive enzyme-linked immunosorbent assay (ELISA). Herein, a chemical staining (CS) strategy was firstly discovered to possess the ability to directly improve the catalytic activity of horseradish peroxidase. Based on this discovery, coomassie brilliant blue was introduced into ELISA to establish a CS enhanced ELISA (CS-ELISA) to detect clenbuterol (CL) by simply staining monoclonal antibodies. Satisfactorily, the most important analytical parameters of CS-ELISA, including sensitivity (0.074 ng mL(-1)) and linear range (0.2-2 ng mL(-1)) were all improving 2-folds compared with conventional ELISA. Moreover, the CS-ELISA shows good applicability in the detection of CL in pork tenderloin samples. The proposed CS-ELISA shows various advantages, such as cost-effective, easily accessible, enhanced catalytic activity of enzyme, higher sensitivity, and broader linear range, providing a new insight into enhanced ELISA for food safety.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61037]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, YC,Zhang, H,Cui, ZW,et al. Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food[J]. FOOD CHEMISTRY,2023,400.
APA	Li, YC.,Zhang, H.,Cui, ZW.,Liu, SJ.,Xu, JK.,...&Wang, JL.(2023).Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food.FOOD CHEMISTRY,400.
MLA	Li, YC,et al."Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food".FOOD CHEMISTRY 400(2023).