For space-based atmospheric wind measurements, full-link simulation is critical for the optimization of the instrument indicators and the evaluation of the measurements' performance. This paper presents observation simulations and error verification of the mesosphere wind measurement with four emission lines of the O-2(0-1) band by using the space-based Doppler Asymmetric Spatial Heterodyne (DASH), named the Mesosphere Wind Image Interferometer (MWII). The passive wind measurement principle and the DASH concept are first described. The full-link simulation consists of radiation simulation, the instrument forward model, and the wind retrieval model. The four emission lines at about 866.5 nm of the O-2(0-1) band were selected as the observation targets. The radiation characteristics of the target lines were studied and calculated, as well as the background radiation. Based on the LOS radiation integral model, a numerical simulation of the raw observation data was carried out using the instrument model. The interference fringe priority strategy and joint wind decision method were proposed to achieve multiple-emission-line wind retrieval with higher precision. In the simulation, multiple-line retrieval could improve the precision by more than 30% compared to single-line retrieval under the same conditions. The error simulation indicated that the wind profile precision was 3-9 m/s in the altitude range of 50-110 km, with an average accuracy of about 1 m/s, proving that the scheme of MWII has good altitude coverage of the whole mesosphere and a part of the lower thermosphere.