Preparation of functionalized magnetic silica nanospheres for the cellulase immobilization

doi:10.1142/S1793292015500137

	Preparation of functionalized magnetic silica nanospheres for the cellulase immobilization
	Zhang, Wenjuan 1; Qiu, Jianhui 1; Zang, Limin 1; Sakai, Eiichi 1; Feng, Huixia 2
刊名	Nano
	2015-01-25
卷号	10 期号:1
关键词	Crosslinking Differential thermal analysis Ethanol Fourier transform infrared spectroscopy High resolution transmission electron microscopy Magnetism Nanospheres Radioactive waste vitrification Reusability Silica Biofuel production cellulase Cross linking agents Functionalized Magnetic silica nanospheres Operational stability Relative activities Vibrating sample magnetometry
ISSN号	17932920
DOI	10.1142/S1793292015500137
英文摘要	Cellulase was immobilized on functionalized magnetic silica nanospheres using glutaraldehyde as a cross-linking agent. The morphologies, structures and magnetic properties of this immobilized cellulase were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, differential thermal analysis and vibrating sample magnetometry. The properties of immobilized cellulase were investigated, including the amount of immobilized cellulase and its relative activity, stability and reusability. The results indicated that immobilized cellulase exhibited better resistance to high temperature and pH inactivation in comparison to free cellulase. Moreover, immobilized cellulase with and without cross-linking agent were investigated and the former had greater amount of immobilized cellulase and better operational stability. The amount of immobilized cellulase with the cross-linking agent was 92 mg/g support. Furthermore, the activity of the immobilized cellulase was still 85.5% of the initial activity after 10 continuous uses, demonstrating the potential of this immobilized cellulase for large-scale biofuel production. © 2015 World Scientific Publishing Company.
WOS研究方向	Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	World Scientific Publishing Co. Pte Ltd
WOS记录号	WOS:000350763300014
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/112859]
专题	教务处（创新创业学院）
作者单位	1.Department of Machine Intelligence and Systems Engineering, Faculty of Systems Engineering, Akita Prefectural University, Akita; 015-0055, Japan; 2.College of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou; 730050, China
推荐引用方式 GB/T 7714	Zhang, Wenjuan,Qiu, Jianhui,Zang, Limin,et al. Preparation of functionalized magnetic silica nanospheres for the cellulase immobilization[J]. Nano,2015,10(1).
APA	Zhang, Wenjuan,Qiu, Jianhui,Zang, Limin,Sakai, Eiichi,&Feng, Huixia.(2015).Preparation of functionalized magnetic silica nanospheres for the cellulase immobilization.Nano,10(1).
MLA	Zhang, Wenjuan,et al."Preparation of functionalized magnetic silica nanospheres for the cellulase immobilization".Nano 10.1(2015).