Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate

doi:10.1007/s12355-016-0473-7

	Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate
	Wang, Yonggang 1,3; Jing, Yanjun 1,3; Leng, Feifan 1,3; Wang, Shiwei 1,3; Wang, Fan 1,3; Zhuang, Yan 1,3; Liu, Xiaofeng 1,3; Wang, Xiaoli 4; Ma, Xueqing 2
刊名	SUGAR TECH
	2017-08
卷号	19 期号:4 页码:424-431
关键词	Microplate Phenol-sulfuric acid colorimetry Glucose Response surface methodology Methodological evaluation
ISSN号	0972-1525
DOI	10.1007/s12355-016-0473-7
英文摘要	To obtain maximal linear regression correlation coefficient (R-2) of glucose standard curve determinations, single-factor test and response surface methodology were applied to optimize conditions including reaction time, incubation temperature and phenol concentration. For investigating optimization of this method, the effects of different metal ions, amino acids and proteins, respectively, were evaluated. The contents of several polysaccharides were determined by three methods, and it was found that the absorption value at 490 nm of the reaction system (230 mu L) placed in the microplate involving 150 mu L sulfuric acid and 30 mu L 5 % phenol solution was in linear relationship with the concentration of glucose range from 10 to 100 nmol/well at 85 degrees C for 20 min. Our results also suggested that the method had a relative deviation of 0.4 %, and the recovery was between 102.7 and 104.10 %.
资助项目	Foundation of Excellent Young Teachers of LUT[10-061406]
WOS研究方向	Agriculture
语种	英语
出版者	SPRINGER INDIA
WOS记录号	WOS:000403439900012
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/33179]
专题	生命科学与工程学院
通讯作者	Wang, Yonggang
作者单位	1.Gansu Educ Dept, Key Lab Screening Evaluat & Adv Proc TCM & Tibeta, Lanzhou 730050, Peoples R China 2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China 3.Lanzhou Univ Technol, Sch Life Sci & Engn, 287 Langongping, Lanzhou 730050, Gansu, Peoples R China 4.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yonggang,Jing, Yanjun,Leng, Feifan,et al. Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate[J]. SUGAR TECH,2017,19(4):424-431.
APA	Wang, Yonggang.,Jing, Yanjun.,Leng, Feifan.,Wang, Shiwei.,Wang, Fan.,...&Ma, Xueqing.(2017).Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate.SUGAR TECH,19(4),424-431.
MLA	Wang, Yonggang,et al."Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate".SUGAR TECH 19.4(2017):424-431.