ISOLATION, CHARACTERISATION, AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI OF Ephedra intermedia

	ISOLATION, CHARACTERISATION, AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI OF Ephedra intermedia
	Xue Hong-Yan; Yang Lin; Wang Yong-Gang; Zhuang Yan; Li Kai-Peng; Li Wei-Juan
刊名	OXIDATION COMMUNICATIONS
	2016
卷号	39 期号:4 页码:2972-2980
关键词	Ephedra intermedia endophytic fungi identification antioxidant activity
ISSN号	0209-4541
英文摘要	6 strains Fusarium, 1 strain Cladosporium, 1 strain Penicillium, 1 strain Pichia kluyveri and 1 strain Alternaria were isolated as endophytic fungi from stems and roots of Ephedra intermedia, based on rDNA sequencing of ITS region. Ethyl acetate extracts of fermented liquid and ethanol extracts of mycelia have been investigated for their reducing power, and scavenging capacity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydroxy radical using in vitro spectrophotometric methods. The results indicate that all the strains fermentation broth showed notable reducing power (more than 100 mu g/mg), three of the fungal strains fermentation broth showed high scavenging activity (scavenging rates more than 70% at a concentration of 0.05 mg/ml), while all extracts from both fermentation broth and mycelia showed poor scavenging capacity against hydroxyl radical, with the highest being that of EI 4 (scavenging rate was 21.17% at a concentration of 0.5 mg/ml). Of the 11 strains, the best overall antioxidant capacity was shown by extracts of EI 2 (Pichia kluyveri). The fermented liquid extract of EI 2 showed a very high reducing power (the equivalent content of vitamin C was 693.36 mu g/mg), an outstanding scavenging ability against DPPH radical (scavenging rate was 59.62% at the concentration of 0.05 mg/ml), whereas showed a moderate hydroxyl radical scavenging activity, with the scavenging rate 14.62% at the concentration of 0.5 mg/ml. The observed results strongly plead further study for development of antioxidant and oxidation drugs from endophytic fungi of Ephedra intermedia has very good prospects.
WOS研究方向	Chemistry
语种	英语
出版者	SCIBULCOM LTD
WOS记录号	WOS:000392409100015
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/155474]
专题	生命科学与工程学院
作者单位	Lanzhou Univ Technol, Sch Life Sci & Engn, Prov Educ Key Lab Screening Evaluat & Adv Proc Tr, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Xue Hong-Yan,Yang Lin,Wang Yong-Gang,et al. ISOLATION, CHARACTERISATION, AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI OF Ephedra intermedia[J]. OXIDATION COMMUNICATIONS,2016,39(4):2972-2980.
APA	Xue Hong-Yan,Yang Lin,Wang Yong-Gang,Zhuang Yan,Li Kai-Peng,&Li Wei-Juan.(2016).ISOLATION, CHARACTERISATION, AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI OF Ephedra intermedia.OXIDATION COMMUNICATIONS,39(4),2972-2980.
MLA	Xue Hong-Yan,et al."ISOLATION, CHARACTERISATION, AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI OF Ephedra intermedia".OXIDATION COMMUNICATIONS 39.4(2016):2972-2980.