Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris

doi:10.2174/0929866524666170426113022

	Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris
	Zhang, Xin-Guo 2,3; Wang, Wen-Na 2,3; Zhang, Chun-Sheng 2,3; Li, Kun 2,3; Ma, Guo-Di 2,3; Li, Jian-Yong 1,4,5
刊名	PROTEIN AND PEPTIDE LETTERS
	2017
卷号	24 期号:7 页码:668-675
关键词	Delta sleep-inducing peptide human serum albumin protein transduction domain Pichia pastoris fusion protein
ISSN号	0929-8665
DOI	10.2174/0929866524666170426113022
英文摘要	Background: Sleep is a natural part of every individual's life. Delta sleep-inducing peptide (DSIP) is a nonapeptide that could promote sleep through the induction of slow wave sleep. However, little is known about the pharmacological effect of DSIP on insomnia. Objectives: The main objective of this study was to analyze the pharmacological effect of DSIP on insomnia. Methods: We designed a fusion protein containing N-terminal TAT-based transduction domain followed by human serum albumin and DSIP and designated this protein as PHD fusion protein. The PHD fusion protein were expressed in Pichia pastoris and purified. Mice were administered single subcutaneous injections three concentrations of PHD fusion protein (0.5, 1, 2 mg/kg), and the pharmacological activity of PHD fusion protein was studied using classic pentobarbital-induced sleep test. Results: We expressed the PHD fusion protein in P. pastoris; furthermore, the PHD fused protein was purified to near homogeneity by DEAE Sepharose FF, Phenyl Sepharose HP and Blue Sepharose 6 FF. Our result showed that the increase of pentobarbital-induced hypnotic effect characterized by reducing sleep latency and prolonged sleep duration was observed for increasing concentrations of PHD fusion protein (P<0.05); moreover, different dose of PHD fusion protein could induce the mice to re-sleep in a dose-dependent manner, whereas higher doses of PHD fusion protein (1.0, 2.0 mg/kg) significantly increased the rate of sleep re-onset compared with the vehicle group of mice (P<0.05). Conclusion: PHD fusion protein increased the hypnotic effects of pentobarbital by reducing sleep latency and prolonged sleep duration. The present study suggested PHD fusion protein could be a new drug candidate for insomnia.
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000410620300011
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/155440]
专题	生命科学与工程学院
作者单位	1.Minist Agr, Key Lab Vet Pharmaceut Dev, Lanzhou 730050, Gansu, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, 287 Langongping Rd, Lanzhou 730050, Gansu, Peoples R China; 3.Key Lab Screening & Proc New Tibetan Med Gansu Pr, Lanzhou 730050, Gansu, Peoples R China; 4.Key Lab New Anim Drug Project Gansu Prov, Lanzhou 730050, Gansu, Peoples R China; 5.Lanzhou Inst Husb & Pharmaceut Sci CAAS, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xin-Guo,Wang, Wen-Na,Zhang, Chun-Sheng,et al. Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris[J]. PROTEIN AND PEPTIDE LETTERS,2017,24(7):668-675.
APA	Zhang, Xin-Guo,Wang, Wen-Na,Zhang, Chun-Sheng,Li, Kun,Ma, Guo-Di,&Li, Jian-Yong.(2017).Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris.PROTEIN AND PEPTIDE LETTERS,24(7),668-675.
MLA	Zhang, Xin-Guo,et al."Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris".PROTEIN AND PEPTIDE LETTERS 24.7(2017):668-675.