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Physical mapping of rDNA and karyotype analysis in Tulipa sinkiangensis and T. schrenkii
Lan, Yue1; Qu, Lianwei2,3; Xin, Haoyang1; Gong, Huiling4; Lei, Jiajun3; Xi, Mengli1
刊名SCIENTIA HORTICULTURAE
2018-10-20
卷号240页码:638-644
关键词Fluorescence in situ hybridization 5S rDNA 45S rDNA Karyotype analysis Tulipa
ISSN号0304-4238
DOI10.1016/j.scienta.2018.06.055
英文摘要The karyotypes of Tulipa sinkiangensis and T. schrenkii were analyzed based on chromosome size and the physical mapping of 5S and 45S ribosomal DNAs (rDNAs) using fluorescence in situ hybridization. The genomes from both species were consistent with the uniform karyotype formula, i.e., having four pairs of submedian and eight pairs of subterminal chromosomes (2n = 2x = 24 = 8sm + 16st). Remarkably, the number and location of 5S rDNA loci were quite different between the two species. A single pair of 5S rDNA loci was detected on both chromosomes 5 in T. sinkiangensis, but the 5S rDNA loci were detected on almost all chromosomes in T. schrenkii, except for chromosomes 4 and 6. For both species, the 45S rDNA loci were localized to telomeric regions of the chromosome-on either the short or long arm. Interestingly, the 5S rDNA and 45S rDNA loci positions and numbers varied among different genotypes of T. schrenkii. We conclude that the two species are close relatives and that a series of genome modifications occurred during the diversification of these two species.
WOS研究方向Agriculture
语种英语
出版者ELSEVIER
WOS记录号WOS:000439763000080
内容类型期刊论文
源URL[http://ir.lut.edu.cn/handle/2XXMBERH/155373]  
专题生命科学与工程学院
作者单位1.Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China;
2.Liaoning Acad Agr Sci, Inst Floriculture, Shenyang 110161, Liaoning, Peoples R China;
3.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Liaoning, Peoples R China;
4.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
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GB/T 7714
Lan, Yue,Qu, Lianwei,Xin, Haoyang,et al. Physical mapping of rDNA and karyotype analysis in Tulipa sinkiangensis and T. schrenkii[J]. SCIENTIA HORTICULTURAE,2018,240:638-644.
APA Lan, Yue,Qu, Lianwei,Xin, Haoyang,Gong, Huiling,Lei, Jiajun,&Xi, Mengli.(2018).Physical mapping of rDNA and karyotype analysis in Tulipa sinkiangensis and T. schrenkii.SCIENTIA HORTICULTURAE,240,638-644.
MLA Lan, Yue,et al."Physical mapping of rDNA and karyotype analysis in Tulipa sinkiangensis and T. schrenkii".SCIENTIA HORTICULTURAE 240(2018):638-644.
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