Microemulsion liquid chromatographic method for simultaneous separation and determination of five isoflavones in red clover

doi:10.1080/10826076.2020.1866599

	Microemulsion liquid chromatographic method for simultaneous separation and determination of five isoflavones in red clover
	Duan, Tianjiao 1,2; Ma, Haixia 2; Dong, Yuming 2; Yang, Fatang 2; Liu, Xiaofeng 1
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2021-02-25
卷号	44 期号:3-4 页码:140-147
关键词	Isoflavones microemulsion liquid chromatography red clover retention mechanism
ISSN号	1082-6076
DOI	10.1080/10826076.2020.1866599
英文摘要	Green and efficient separation methods are gradually being loved, and microemulsion phase chromatography is used in many fields. This study used a microemulsion liquid chromatographic method for the simultaneous separation of five isoflavones (formononetin, ononin, calycosin, calycosin-7-glucoside, and biochanin A) in red clover within 10 minutes for the first time. Separation and analysis are performed at GS-120-5-C18-AP column with UV detection at 254 nm. The mobile phase was made up of 5% SDS(w/v), 7% n-butyl alcohol (w/v), 0.9% octane (w/v), 87.1% H2O (w/v), pH adjusted to 3.0 with 30 mM phosphoric acid. Under this optimal condition, the linear range of the calibration curve of the five isoflavones is 1-50 mu g/ml and the correlation coefficients are greater than 0.9973. Limit of detection (0.18-0.4 mu g/ml), limit of quantitation (0.41-0.97 mu g/ml), accuracy (82.4-98.6%) and the intra and inter precision (RSD <3.3%, and <5.6%). Method developed in this study is simple, low cost, green, efficient and provides an optional method for the speedy isolation of red clover isoflavones in 10 minutes, and it is not necessary to set a complex gradient method. [GRAPHICS] .
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000607683900001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148849]
专题	生命科学与工程学院
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China; 2.Lanzhou Univ, Sch Pharm, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Duan, Tianjiao,Ma, Haixia,Dong, Yuming,et al. Microemulsion liquid chromatographic method for simultaneous separation and determination of five isoflavones in red clover[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2021,44(3-4):140-147.
APA	Duan, Tianjiao,Ma, Haixia,Dong, Yuming,Yang, Fatang,&Liu, Xiaofeng.(2021).Microemulsion liquid chromatographic method for simultaneous separation and determination of five isoflavones in red clover.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,44(3-4),140-147.
MLA	Duan, Tianjiao,et al."Microemulsion liquid chromatographic method for simultaneous separation and determination of five isoflavones in red clover".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 44.3-4(2021):140-147.