Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids

doi:10.1007/s11696-019-00791-0

	Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids
	Ke, Jing 1; Duan, Xiao-Yun 2; Liu, Xiao-Feng 2; Dong, Yu-Ming 1
刊名	CHEMICAL PAPERS
	2019-10
卷号	73 期号:10 页码:2417-2426
关键词	Amino acids Micellar liquid chromatography Mixed surfactants Polyoxyethylene lauryl ether Sodium dodecyl sulfate
ISSN号	2585-7290
DOI	10.1007/s11696-019-00791-0
英文摘要	In this work, an isocratic micellar chromatographic method for separation of 17 free amino acids was first established. Mixed anionic and non-ionic surfactants were used as mobile phase additives. Besides, a chromatographic environment was simulated and the possible mechanism was discussed. Pre-column derivatization with 9-fluorenylmethyl chloroformate was used before chromatographic analysis. The optimized chromatographic conditions were the mobile phase A (0.075M sodium dodecyl sulfate solutions and 0.010M polyoxyethylene lauryl ether containing 20mM ammonium acetate at pH 3.5) and B (100% acetonitrile) (85:15, v/v) running at 1.2mL/min by a Venusil XBPC18 column (5 mu m, 250x4.6mm) at 35.0 degrees C. Compared with the conventional reversed phase liquid chromatography, the consumption of organic solvent in the method is lower which reduces the analysis cost and being environment-friendly. In addition, complex gradient elution is not required. This provides an alternative way for the separation of amino acids.
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER INTERNATIONAL PUBLISHING AG
WOS记录号	WOS:000474390300005
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/31529]
专题	生命科学与工程学院
作者单位	1.Lanzhou Univ, Sch Pharm, Inst Pharmaceut Anal, Lanzhou 730000, Gansu, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Ke, Jing,Duan, Xiao-Yun,Liu, Xiao-Feng,et al. Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids[J]. CHEMICAL PAPERS,2019,73(10):2417-2426.
APA	Ke, Jing,Duan, Xiao-Yun,Liu, Xiao-Feng,&Dong, Yu-Ming.(2019).Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids.CHEMICAL PAPERS,73(10),2417-2426.
MLA	Ke, Jing,et al."Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids".CHEMICAL PAPERS 73.10(2019):2417-2426.