Long-term nitrogen and phosphorus removal, shifts of functional bacteria and fate of resistance genes in bioretention systems under sulfamethoxazole stress

doi:10.1016/j.jes.2022.03.045

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	Long-term nitrogen and phosphorus removal, shifts of functional bacteria and fate of resistance genes in bioretention systems under sulfamethoxazole stress
	Xu, Yan 2; Zhang, Danyi 2; Xue, Qingju 3; Bu, Chibin 4; Wang, Yajun 1; Zhang, Benchi 5; Wang, Ying 2; Qin, Qingdong 2
刊名	Journal of Environmental Sciences (China)
	2023-04-01
卷号	126 页码:1-16
关键词	Bacteria Effluents Genes Iron Nitrogen Nitrogen removal Nutrients Phosphorus Shells (structures) Substrates Antibiotic-resistant genes Bioretention Bioretention system Coconut shells Coconut-shell activated carbon Functional bacteria Nitrogen and phosphorus removal Resistance genes Sulfamethoxazole Zero-valent iron
ISSN号	1001-0742
DOI	10.1016/j.jes.2022.03.045
英文摘要	To understand the long-term performance of bioretention systems under sulfamethoxazole (SMX) stress, an unplanted bioretention system (BRS) and two modified BRSs with coconut-shell activated carbon (CAC) and CAC/zero-valent-iron (Fe0) granules (CAC-BRS and Fe/CAC-BRS) were established. Both CAC-BRS and Fe/CAC-BRS significantly outperformed BRS in removing total nitrogen (TN) (CAC-BRS: 82.48%; Fe/CAC-BRS: 78.08%; BRS: 47.51%), total phosphorous (TP) (CAC-BRS: 79.36%; Fe/CAC-BRS: 98.26%; BRS: 41.99%), and SMX (CAC-BRS: 99.74%, Fe/CAC-BRS: 99.80%; BRS: 23.05%) under the long-term SMX exposure (0.8 mg/L, 205 days). High-throughput sequencing revealed that the microbial community structures of the three BRSs shifted greatly in upper zones after SMX exposure. Key functional genera, dominantly Nitrospira, Rhodoplanes, Desulfomicrobium, Geobacter, were identified by combining the functional prediction by the FAPROTAX database with the dominant genera. The higher abundance of nitrogen functional genes (nirK, nirS and nosZ) in CAC-BRS and Fe/CAC-BRS might explain the more efficient TN removal in these two systems. Furthermore, the relative abundance of antibiotic-resistant genes (ARGs) sulI and sulII increased in all BRSs along with SMX exposure, suggesting the selection of bacteria containing sul genes. Substrates tended to become reservoirs of sul genes. Also, co-occurrence network analysis revealed distinct potential host genera of ARGs between upper and lower zones. Notably, Fe/CAC-BRS succeeded to reduce the effluent sul genes by 1-2 orders of magnitude, followed by CAC-BRS after 205-day exposure. This study demonstrated that substrate modification was crucial to maintain highly efficient nutrients and SMX removals, and ultimately extend the service life of BRSs in treating SMX wastewater. © 2022
语种	英语
出版者	Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/159126]
专题	土木工程学院
作者单位	1.School of Civil Engineering, Lanzhou University of Technology, Lanzhou; 730050, China; 2.Department of Municipal Engineering, School of Civil Engineerin, Southeast University, Nanjing; 210096, China; 3.Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences (NIGLAS), Nanjing; 210008, China; 4.Department of Gastroenterology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing; 210096, China; 5.Department of Environmental Systems Engineering, University of Regina, Regina; SK S4S0A2, Canada
推荐引用方式 GB/T 7714	Xu, Yan,Zhang, Danyi,Xue, Qingju,et al. Long-term nitrogen and phosphorus removal, shifts of functional bacteria and fate of resistance genes in bioretention systems under sulfamethoxazole stress[J]. Journal of Environmental Sciences (China),2023,126:1-16.
APA	Xu, Yan.,Zhang, Danyi.,Xue, Qingju.,Bu, Chibin.,Wang, Yajun.,...&Qin, Qingdong.(2023).Long-term nitrogen and phosphorus removal, shifts of functional bacteria and fate of resistance genes in bioretention systems under sulfamethoxazole stress.Journal of Environmental Sciences (China),126,1-16.
MLA	Xu, Yan,et al."Long-term nitrogen and phosphorus removal, shifts of functional bacteria and fate of resistance genes in bioretention systems under sulfamethoxazole stress".Journal of Environmental Sciences (China) 126(2023):1-16.