In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets

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	In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets
	Tang, W
刊名	PLANT CELL REPORTS
	2001-02-01
卷号	20 期号:2 页码:163-168
关键词	Pinus taeda L. organogenesis plant regeneration random amplified polymorphic DNA
ISSN号	0721-7714
其他题名	Plant Cell Reports
中文摘要	Adventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.
英文摘要	Adventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	MATURE ZYGOTIC EMBRYOS ; RAPD MARKERS ; SOMATIC EMBRYOGENESIS ; SOMACLONAL VARIATION ; CALLUS-CULTURES ; TAEDA ; ORGANOGENESIS ; CONIFERS ; L.
收录类别	SCI
原文出处	://WOS:000167189500010
语种	英语
WOS记录号	WOS:000167189500010
公开日期	2013-11-14
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5751]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.N Carolina State Univ, Dept Forestry, Forestry Biotechnol Grp, Raleigh, NC 27695 USA 2.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Tang, W. In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets[J]. PLANT CELL REPORTS,2001,20(2):163-168.
APA	Tang, W.(2001).In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.PLANT CELL REPORTS,20(2),163-168.
MLA	Tang, W."In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets".PLANT CELL REPORTS 20.2(2001):163-168.