Promoting Resuscitation of the VBNC Bacteria and Enrichment of Naphthalene-degrading Bacteria from Oil-contaminated Soils with the Rpf4 from Rhodococcus erythropolis KB1

doi:10.1080/01490451.2022.2097339

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	Promoting Resuscitation of the VBNC Bacteria and Enrichment of Naphthalene-degrading Bacteria from Oil-contaminated Soils with the Rpf4 from Rhodococcus erythropolis KB1
	Wang, Xiaoning 3; Chen, Jixiang 3; Wang, Yonggang 2; Li, YunChun 1; Chen, ShiYuan 3; Zhang, Qingfang 3
刊名	GEOMICROBIOLOGY JOURNAL
	2022-07-01
关键词	Diversity naphthalene-degrading bacteria resuscitation resuscitation-promoting factors Rhodococcus erythropolis
ISSN号	0149-0451
DOI	10.1080/01490451.2022.2097339
英文摘要	Nowadays, more than 90% of bacteria in nature are in a viable but not culturable (VBNC) state, and they cannot be separated by traditional plate culture methods. However, resuscitation-promoting factors (Rpf) participate in recovery of dormant cells and promoting bacterial growth. The Rpf4 of Rhodococcus erythropolis KB1 is a Rpf like protein of Micrococcus luteus and contained the Rpf domain and LysM domain. In this study, an expression vector for the rpf4 gene was constructed and expressed in Escherichia coli. The recombinant protein Rpf4 was purified by Ni2+-affinity chromatography. The recombinant protein showed a muralytic activity of 549 +/- 28.77 U/mg. The purified Rpf4 could increase R. erythropolis KB1 cell proliferation and improve the resuscitation of R. erythropolis cells that were VBNC. The Rpf4 altered the diversity of naphthalene-degrading bacteria enriched from oil-contaminated soils. Based on the high-throughput sequencing, we found that Proteobacteria and Actinobacteria phyla increased from 76.10% and 0.029% to 98.23% and 0.401% after the Rpf4 treatment, while the Bacteroidetes phylum decreased from 22.70% to 0.76%. The genera Enterobacter, Citrobacter, and Acinetobacter increased from 0.0054%, 0.001%, and 0.745% to 19.02%, 6.34% and 2.49%. Some new genera such as Bifidobacterium, Limnobacter, Alloprevotella, Hydrogenophaga, and Nevskia were founded in the treatment group. Meanwhile, 23 naphthalene-degrading bacteria were isolated by adding the Rpf4 protein to the oil-contaminated soil samples. Study on the naphthalene-degrading bacteria will provide a basis for the microbial degradation of naphthalene and other polycyclic aromatic hydrocarbons and discover the wider application value of Rpf.
WOS研究方向	Environmental Sciences & Ecology ; Geology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000828271500001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/159461]
专题	石油化工学院生命科学与工程学院
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou, Peoples R China 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China; 3.Lanzhou Univ Technol, Sch Petrochem Engn, 36 Pengjiaping Rd, Lanzhou 730050, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Xiaoning,Chen, Jixiang,Wang, Yonggang,et al. Promoting Resuscitation of the VBNC Bacteria and Enrichment of Naphthalene-degrading Bacteria from Oil-contaminated Soils with the Rpf4 from Rhodococcus erythropolis KB1[J]. GEOMICROBIOLOGY JOURNAL,2022.
APA	Wang, Xiaoning,Chen, Jixiang,Wang, Yonggang,Li, YunChun,Chen, ShiYuan,&Zhang, Qingfang.(2022).Promoting Resuscitation of the VBNC Bacteria and Enrichment of Naphthalene-degrading Bacteria from Oil-contaminated Soils with the Rpf4 from Rhodococcus erythropolis KB1.GEOMICROBIOLOGY JOURNAL.
MLA	Wang, Xiaoning,et al."Promoting Resuscitation of the VBNC Bacteria and Enrichment of Naphthalene-degrading Bacteria from Oil-contaminated Soils with the Rpf4 from Rhodococcus erythropolis KB1".GEOMICROBIOLOGY JOURNAL (2022).