Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups

doi:10.1007/s42114-019-00099-3

	Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups
	Zhang, Xiayun 1,2,3; Chen, Zhenbin 1,2; Li, Ke 1,2; Yang, Zhongduo 3; Li, Zhizhong 3; Xie, Dengmin 1,2; Zhou, Wenming 1,2; Wang, Ting 1,2; Ma, Songmei 4; Burns, Robert 5
刊名	ADVANCED COMPOSITES AND HYBRID MATERIALS
	2019-12-01
卷号	2 期号:4 页码:720-734
关键词	Fe3O4 nanoparticles Modification Composite carrier Penicillin G Acylase Superparamagnetic
ISSN号	2522-0128
DOI	10.1007/s42114-019-00099-3
英文摘要	In this work, Fe3O4 particles were first prepared by a reversed microemulsion method, and then the prepared nanoparticles were modified with 3-(trimethoxysilyl) propyl methacrylate (KH-570), a silane coupling agent, to enhance the dispersibility and biocompatibility of Fe3O4 particles. To get the graft ratio quantitatively, and further achieve the maximum loading capacity of KH-570, the characterization method for the graft ratio (Gr) was systemically explored first; reformative silicon molybdenum chromogenic (RSMC) was designed and presented a good result. In addition, the influence of operation conditions on the graft ratio was investigated and optimized. Results showed the graft ratio of KH-570 on magnetic Fe3O4 nanoparticles arrived at 178.51% under optimal conditions. In addition, it displayed KH-570 grafted onto the surface of Fe3O4 nanoparticles successfully, and only one silicon hydroxyl was bonded on the surface of Fe3O4 nanoparticles under optimal modifications. Finally, a magnetic composite carrier was prepared by grafting glycidyl methacrylate (GMA) onto the surface of the modified nanoparticles. Further, the composite was used to immobilize penicillin G acylase (PGA); in addition, the effect of GMA dosage on enzyme activity (EA), enzyme activity recovery rate (EAR), and enzyme loading rate (ELR) was studied. It was found that when the amount of GMA was 9.0%, the EA, EAR, and ELR were 9208 U/g, 88 mg/g, and 64.08%, respectively. In addition, the immobilized PGA still possess superparamagnetism, which can be well recycled and reused.
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	SPRINGERNATURE
WOS记录号	WOS:000589403600011
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/155279]
专题	材料科学与工程学院机电工程学院生命科学与工程学院
作者单位	1.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 4.Ludong Univ, Sch Chem & Mat Sci, Yantai 264025, Peoples R China; 5.Frostburg State Univ, Dept Phys & Engn, Frostburg, MD 21532 USA; 6.Univ Santiago de Compostela, Soft Matter & Mol Biophys Grp, Dept Appl Phys, Santiago De Compostela 15782, Spain; 7.South China Univ Technol, Guangzhou Higher Educ Mega Ctr, Guangzhou Key Lab Surface Chem Energy Mat, New Energy Res Inst,Sch Environm & Energy, Guangzhou 5100067, Peoples R China; 8.South China Univ Technol, Guangdong Prov Key Lab Atmospher Environm & Pollu, Guangdong Prov Engn & Technol Res Ctr Environm Ri, Sch Environm & Energy,Guangzhou Higher Educ Mega, Guangzhou 5100067, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiayun,Chen, Zhenbin,Li, Ke,et al. Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups[J]. ADVANCED COMPOSITES AND HYBRID MATERIALS,2019,2(4):720-734.
APA	Zhang, Xiayun.,Chen, Zhenbin.,Li, Ke.,Yang, Zhongduo.,Li, Zhizhong.,...&Liu, Zhen.(2019).Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups.ADVANCED COMPOSITES AND HYBRID MATERIALS,2(4),720-734.
MLA	Zhang, Xiayun,et al."Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups".ADVANCED COMPOSITES AND HYBRID MATERIALS 2.4(2019):720-734.