Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli

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	Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli
	Wang, Dan 1,2; Li, Qiang 1,2; Li, Wangliang 1; Xing, Jianmin 1; Su, Zhiguo 1
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2009-12-01
卷号	45 期号:6-7 页码:491-497
关键词	Succinate Escherichia coli Carbonic anhydrase (CA) Bicarbonate Carbon dioxide
ISSN号	0141-0229
英文摘要	Succinate fermentation was investigated in Escherichia coli strains overexpressing cyanobacterium Anabaena sp. 7120 ecaA gene encoding carbonic anhydrase (CA). In strain BL21 (DE3) bearing ecaA, the activity of CA was 21.8 U mg(-1) protein, whereas non-detectable CA activity was observed in the control strain. Meanwhile. the activity of phosphoenolpyruvate carboxylase (PEPC) increased from 0 2 U mg(-1) protein to 1.13 U mg(-1) protein. The recombinant bearing ecaA reached a succinate yield of 0.39 mol mol(-1) in glucose at the end of the fermentation. It was 21-fold higher than that of con trot strain which was Just 0 19 mol mol(-1) glucose EcaA gene was also introduced into E coli DC1515, which was deficient in glucose phosphotransferase. lactate dehydrogenase and pyruvate formate lyase succinate yield call be further increased to 1 26 mol mol(-1) glucose It could be concluded that the enhancement of the supply of HCO3- in vivo by ecaA overexpression is all effective strategy for the Improvement of succinate production in E. Coli. (C) 2009 Elsevier Inc All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	PHOSPHOENOLPYRUVATE CARBOXYKINASE ; PYRUVATE-CARBOXYLASE ; ACID PRODUCTION ; MANNHEIMIA-SUCCINICIPRODUCENS ; LACTATE-DEHYDROGENASE ; MALIC ENZYME ; BOVINE RUMEN ; FERMENTATION ; EXPRESSION ; CO2
收录类别	SCI
语种	英语
WOS记录号	WOS:000271260700013
公开日期	2013-10-29
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4370]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Dan,Li, Qiang,Li, Wangliang,et al. Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli[J]. ENZYME AND MICROBIAL TECHNOLOGY,2009,45(6-7):491-497.
APA	Wang, Dan,Li, Qiang,Li, Wangliang,Xing, Jianmin,&Su, Zhiguo.(2009).Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli.ENZYME AND MICROBIAL TECHNOLOGY,45(6-7),491-497.
MLA	Wang, Dan,et al."Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli".ENZYME AND MICROBIAL TECHNOLOGY 45.6-7(2009):491-497.