Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex

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	Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex
	Zhang, SP ; Norrlow, O ; Dey, ES
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2005-10-01
卷号	71 期号:10 页码:5888-5892
关键词	agilis cells immobilized cells substrate consumption calcium alginate activated-sludge oxygen carriers nitrite model nitrification carrageenan
ISSN号	0099-2240
其他题名	Appl. Environ. Microbiol.
中文摘要	Removal of nitrite and nitrate from drinking water has attracted great attention in recent years because of the human health risk induced by the exposure to contaminated groundwater and surface water. We have therefore tested a model nitrite oxidation system by coentrapping the NaNO2 oxidizer Nitrobacter vulgaris with polydimethylsiloxane (PDMS) copolymer and DEAE-Sephadex in a polyacrylamide gel. The copolymer and the anion exchanger facilitate the diffusion of oxygen and NaNO2, respectively, into the gel matrix. To test the nitrite-oxidizing activity, the entrapped cells were coupled to a thermal sensor. Coentrapment of 5% (wt/vol) DEAE-Sephadex with Nitrobacter vulgaris increased the nitrite-oxidizing activity by a factor of 3.7 compared to entrapped cells alone, and by the addition of 0.86% (wt/vol) artificial oxygen carrier PDMS copolymer increased the activity further to 4.3 times higher. Operational and storage stability of the coentrapped N. vulgaris also improved. This suggests that this enhanced immobilized cell system can also be used for nitrite oxidation to nitrate in drinking water as an on-line thermally monitored bioreactor.
英文摘要	Removal of nitrite and nitrate from drinking water has attracted great attention in recent years because of the human health risk induced by the exposure to contaminated groundwater and surface water. We have therefore tested a model nitrite oxidation system by coentrapping the NaNO2 oxidizer Nitrobacter vulgaris with polydimethylsiloxane (PDMS) copolymer and DEAE-Sephadex in a polyacrylamide gel. The copolymer and the anion exchanger facilitate the diffusion of oxygen and NaNO2, respectively, into the gel matrix. To test the nitrite-oxidizing activity, the entrapped cells were coupled to a thermal sensor. Coentrapment of 5% (wt/vol) DEAE-Sephadex with Nitrobacter vulgaris increased the nitrite-oxidizing activity by a factor of 3.7 compared to entrapped cells alone, and by the addition of 0.86% (wt/vol) artificial oxygen carrier PDMS copolymer increased the activity further to 4.3 times higher. Operational and storage stability of the coentrapped N. vulgaris also improved. This suggests that this enhanced immobilized cell system can also be used for nitrite oxidation to nitrate in drinking water as an on-line thermally monitored bioreactor.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	AGILIS CELLS ; IMMOBILIZED CELLS ; SUBSTRATE CONSUMPTION ; CALCIUM ALGINATE ; ACTIVATED-SLUDGE ; OXYGEN CARRIERS ; NITRITE ; MODEL ; NITRIFICATION ; CARRAGEENAN
收录类别	SCI
原文出处	://WOS:000232504000028
语种	英语
WOS记录号	WOS:000232504000028
公开日期	2013-10-25
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4176]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Lund Univ, S-22100 Lund, Sweden 2.Kemira Kemi AB, Recycling Comptence Ctr, S-25109 Helsingborg, Sweden 3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhang, SP,Norrlow, O,Dey, ES. Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2005,71(10):5888-5892.
APA	Zhang, SP,Norrlow, O,&Dey, ES.(2005).Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,71(10),5888-5892.
MLA	Zhang, SP,et al."Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 71.10(2005):5888-5892.