Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method

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	Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method
	Hu, Hanhua ; Shi, Yanjun ; Cong, Wei
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2006-04-01
卷号	18 期号:2 页码:119-126
关键词	airlift photobioreactor Alexandrium tamarense growth saxitoxin two-step culture
ISSN号	0921-8971
其他题名	J. Appl. Phycol.
中文摘要	The growth and toxin content of the dinoflagellate Alexandrium tamarense ATHK was markedly affected by culture methods. In early growth phase at lower cell density static or mild agitation methods were beneficial to growth, but continuous agitation or aeration, to some extent, had an adverse effect on cell growth. Static culture in 2 L Erlenmeyer flasks had the highest growth rate (0.38 d(-1)) but smaller cell size compared with other culture conditions. Cells grown under aerated conditions possessed low nitrogen and phosphorus cell yields, namely high N and P cell-quota. At day 18, cells grown in continuous agitated and 1 h aerated culture entered the late stationary phase and their cellular toxin contents were higher (0.67 and 0.54 pg cell(-1)) compared with cells grown by other culture methods (0.27-0.49 pg cell(-1)). The highest cell density and cellular toxin content were 17190 cells mL(-1) and 1.26 pg cell(-1) respectively in an airlift photobioreactor with two-step culture. The results indicate that A. tamarense could be grown successfully in airlift photobioreactor by a two-step culture method, which involved cultivating the cells statically for 4 days and then aerating the medium. This provides an efficient way to enhance cell and toxin yield of A. tamarense.
英文摘要	The growth and toxin content of the dinoflagellate Alexandrium tamarense ATHK was markedly affected by culture methods. In early growth phase at lower cell density static or mild agitation methods were beneficial to growth, but continuous agitation or aeration, to some extent, had an adverse effect on cell growth. Static culture in 2 L Erlenmeyer flasks had the highest growth rate (0.38 d(-1)) but smaller cell size compared with other culture conditions. Cells grown under aerated conditions possessed low nitrogen and phosphorus cell yields, namely high N and P cell-quota. At day 18, cells grown in continuous agitated and 1 h aerated culture entered the late stationary phase and their cellular toxin contents were higher (0.67 and 0.54 pg cell(-1)) compared with cells grown by other culture methods (0.27-0.49 pg cell(-1)). The highest cell density and cellular toxin content were 17190 cells mL(-1) and 1.26 pg cell(-1) respectively in an airlift photobioreactor with two-step culture. The results indicate that A. tamarense could be grown successfully in airlift photobioreactor by a two-step culture method, which involved cultivating the cells statically for 4 days and then aerating the medium. This provides an efficient way to enhance cell and toxin yield of A. tamarense.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	SMALL-SCALE TURBULENCE ; PHOSPHORUS LIMITATION ; NATURAL PHYTOPLANKTON ; C2 TOXIN ; DINOFLAGELLATE ; DINOPHYCEAE ; TOXICITY ; MINUTUM ; NITRATE ; INCREASE
收录类别	SCI
原文出处	://WOS:000239891700002
语种	英语
WOS记录号	WOS:000239891700002
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3992]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China 2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Hu, Hanhua,Shi, Yanjun,Cong, Wei. Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method[J]. JOURNAL OF APPLIED PHYCOLOGY,2006,18(2):119-126.
APA	Hu, Hanhua,Shi, Yanjun,&Cong, Wei.(2006).Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method.JOURNAL OF APPLIED PHYCOLOGY,18(2),119-126.
MLA	Hu, Hanhua,et al."Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method".JOURNAL OF APPLIED PHYCOLOGY 18.2(2006):119-126.