A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography

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	A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography
	Qu, Jian-Bo 1; Huang, Yong-Dong 2; Jing, Guang-Lun 1; Liu, Jian-Guo 1; Zhou, Wei-Qing 2; Zhu, Hu 1; Lu, Jian-Ren 1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2011-05-01
卷号	879 期号:15-16 页码:1043-1048
关键词	Gigaporous Polystyrene microspheres Column efficiency Immobilized-metal affinity chromatography Superoxide dismutase
ISSN号	1570-0232
通讯作者	Qu, JB
英文摘要	Agarose coated gigaporous polystyrene microspheres were evaluated as a novel matrix for immobilized-metal affinity chromatography (IMAC). With four steps, nickel ions were successfully immobilized on the microspheres. The gigaporous structure and chromatographic properties of IMAC medium were characterized. A column packed with the matrix showed low column backpressure and high column efficiency at high flow velocity. Furthermore, this matrix was used for purifying superoxide dismutase (SOD), which was expressed in Escherichia coli (E. coli) in submerged fermentation, on an Akta purifier 100 system under different flow velocities. The purity of the SOD from this one-step purification was 79% and the recovery yield was about 89.6% under the superficial flow velocity of 3251 cm/h. In conclusion, all the results suggested that the gigaporous matrix has considerable advantages for high-speed immobilized-metal affinity chromatography. (C) 2011 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	PERFUSION CHROMATOGRAPHY ; PROTEIN CHROMATOGRAPHY ; PURIFICATION ; SEPARATION ; DISMUTASE ; MEDIA
收录类别	SCI
语种	英语
WOS记录号	WOS:000290645400003
公开日期	2013-11-05
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4882]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Qu, Jian-Bo,Huang, Yong-Dong,Jing, Guang-Lun,et al. A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(15-16):1043-1048.
APA	Qu, Jian-Bo.,Huang, Yong-Dong.,Jing, Guang-Lun.,Liu, Jian-Guo.,Zhou, Wei-Qing.,...&Lu, Jian-Ren.(2011).A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(15-16),1043-1048.
MLA	Qu, Jian-Bo,et al."A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.15-16(2011):1043-1048.