Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface

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	Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface
	Jiang, Yangyang 1,2; Guo, Chen 1; Gao, Hongshuai 1; Xia, Hansong 1; Mahmood, Iram 1; Liu, Chunzhao 1; Liu, Huizhou 1
刊名	AICHE JOURNAL
	2012-04-01
卷号	58 期号:4 页码:1203-1211
关键词	magnetic nanoparticles ionic liquids lipase oil-water two-phase ester hydrolysis
ISSN号	0001-1541
通讯作者	Guo, C
英文摘要	Ester hydrolysis at oilwater interface by lipase covalently immobilized on ionic liquid-modified magnetic nanoparticles was investigated. Magnetic supports with a diameter of 1015 nm were synthesized by covalent binding of ionic liquids (chain length C4 and C8 and anions Cl-, BF4-, and PF6-) on the surface of Fe3O4 nanoparticles. Lipase was covalently immobilized on Fe3O4 nanoparticles using ionic liquids as the coupling reagent. Ionic liquid-modified magnetic nanoparticle-grafted lipase preferentially located at the oilwater interface. It has higher catalytic activity than its native counterpart. A modified MichaelisMenten model was used to elucidate the effect of stirring rate, aqueousorganic phase ratio, total amount of enzyme, and ester chain length. The influences of these conditions on esters hydrolysis at oilwater interface were consistent with the introduction of the ionic liquids interlayer. Ionic liquids could be used to control the oilwater interfacial characteristics during lipase catalyzed hydrolysis, and thus control the behavior of immobilized lipase. (C) 2011 American Institute of Chemical Engineers AIChE J, 2012
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Chemical
研究领域[WOS]	Engineering
关键词[WOS]	CANDIDA-RUGOSA LIPASE ; ENZYMATIC-HYDROLYSIS ; ORGANIC-SOLVENTS ; SILICA ; SYSTEMS ; CATALYSIS ; KINETICS ; DYE
收录类别	SCI
语种	英语
WOS记录号	WOS:000301225700018
公开日期	2013-10-23
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3880]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China 2.Nanjing Chem Ind Grp, Res Inst, Nanjing 210048, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yangyang,Guo, Chen,Gao, Hongshuai,et al. Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface[J]. AICHE JOURNAL,2012,58(4):1203-1211.
APA	Jiang, Yangyang.,Guo, Chen.,Gao, Hongshuai.,Xia, Hansong.,Mahmood, Iram.,...&Liu, Huizhou.(2012).Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface.AICHE JOURNAL,58(4),1203-1211.
MLA	Jiang, Yangyang,et al."Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface".AICHE JOURNAL 58.4(2012):1203-1211.