Exposure Duration Is a Determinant of the Effect of Sinusoidal Electromagnetic Fields on Peak Bone Mass of Young Rats | |
Zhu, B. Y.1,2; Yang, Z. D.1; Chen, X. R.3; Zhou, J.2; Gao, Y. H.2; Xian, C. J.4; Chen, K. M.2 | |
刊名 | CALCIFIED TISSUE INTERNATIONAL |
2018-07 | |
卷号 | 103期号:1页码:95-106 |
关键词 | Sinusoidal electromagnetic fields Exposure duration Osteoblasts Peak bone mass Bone mineral density |
ISSN号 | 0171-967X |
DOI | 10.1007/s00223-018-0396-2 |
英文摘要 | We proposed a three-step strategy to obtain the optimal therapeutic parameters, which is composed of large-scale screening at cellular level, verification in animal experiments, and confirmation by a clinical trial. The objective of the current study was to test the feasibility of our strategy. Newborn rat calvarial osteoblasts were treated by 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) with 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 h/days, respectively. The osteogenic differentiation and maturation of the osteoblast were assayed and compared to obtain the optimal duration. One-month-old growing rats were then treated by the same SEMFs with 0.5, 1.5, and 2.5 h/days, respectively, and the peak bone mass was analyzed after 2 months. It was found that the optimal exposure duration to promote the osteogenic differentiation and maturation of osteoblasts was 1.5 h/days, judging by the increasing degrees of ALP activity, calcified nodules formed, the gene and protein expression levels of Runx-2, BMP-2, and Col-I, as well as the expression levels of signaling proteins of the BMP-2/Smad1/5/8 pathway. The highest increase of peak bone mass after 2 months was also obtained by 1.5 h/days, judging by the results of X-ray dual-energy absorptiometry, mechanical property analysis, micro-CT scanning, and serum bone turnover marker examinations. The above results indicated that exposure duration is a determinant for the therapeutic effect of EMFs, and the optimal therapeutic effects only can be obtained by the optimal exposure duration. |
资助项目 | NHMRC Australia[1042105] |
WOS研究方向 | Endocrinology & Metabolism |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000435779600012 |
状态 | 已发表 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.223/handle/2XXMBERH/32591] |
专题 | 兰州理工大学 |
通讯作者 | Yang, Z. D.; Chen, K. M. |
作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 2.CPLA, Lanzhou Gen Hosp, Inst Orthopaed, Lanzhou 730050, Gansu, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yanglin 712100, Peoples R China 4.Univ South Australia, Sansom Inst Hlth Res, Sch Pharm & Med Sci, Adelaide, SA 5001, Australia |
推荐引用方式 GB/T 7714 | Zhu, B. Y.,Yang, Z. D.,Chen, X. R.,et al. Exposure Duration Is a Determinant of the Effect of Sinusoidal Electromagnetic Fields on Peak Bone Mass of Young Rats[J]. CALCIFIED TISSUE INTERNATIONAL,2018,103(1):95-106. |
APA | Zhu, B. Y..,Yang, Z. D..,Chen, X. R..,Zhou, J..,Gao, Y. H..,...&Chen, K. M..(2018).Exposure Duration Is a Determinant of the Effect of Sinusoidal Electromagnetic Fields on Peak Bone Mass of Young Rats.CALCIFIED TISSUE INTERNATIONAL,103(1),95-106. |
MLA | Zhu, B. Y.,et al."Exposure Duration Is a Determinant of the Effect of Sinusoidal Electromagnetic Fields on Peak Bone Mass of Young Rats".CALCIFIED TISSUE INTERNATIONAL 103.1(2018):95-106. |
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