Identification and expression profile of Id1 in bighead carp in response to microcystin-LR

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	Identification and expression profile of Id1 in bighead carp in response to microcystin-LR
	Li, Huiying; Cai, Yan; Xie, Ping; Chen, Jun; Hao, Le; Li, Guangyu; Xiong, Qian
刊名	ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
	2012-09-01
卷号	34 期号:2 页码:324-333
关键词	Id1 Gene identification mRNA expression Microcystin-LR Bighead carp
ISSN号	1382-6689
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
中文摘要	Microcystin-LR (MCLR) is a widespread cyanotoxin produced in algal blooms, and has potent hepatotoxicity and tumor-promoting activity. We cloned the full-length cDNA of Id1 in bighead carp. The full-length Id1 cDNA was 954 bp and contained a 387 bp ORF. Bighead carp Id1 shared high identity with zebrafish Id1 amino acid sequence, and phylogenetic analysis showed that teleost Id1 evolved closely. Bighead carp Id1 constitutively expressed in all tested tissues in normal. When tested at two different time points post exposure and at 3 different MCLR doses, Id1 expression increased in a time-dependent pattern, and Id1 expression in brain was very sensitive to MCLR exposure. The present study will help us to understand more about the evolution of Id1 molecule and its role in the MCLR induced cell differentiation and cancer promoting in bighead carp. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
英文摘要	Microcystin-LR (MCLR) is a widespread cyanotoxin produced in algal blooms, and has potent hepatotoxicity and tumor-promoting activity. We cloned the full-length cDNA of Id1 in bighead carp. The full-length Id1 cDNA was 954 bp and contained a 387 bp ORF. Bighead carp Id1 shared high identity with zebrafish Id1 amino acid sequence, and phylogenetic analysis showed that teleost Id1 evolved closely. Bighead carp Id1 constitutively expressed in all tested tissues in normal. When tested at two different time points post exposure and at 3 different MCLR doses, Id1 expression increased in a time-dependent pattern, and Id1 expression in brain was very sensitive to MCLR exposure. The present study will help us to understand more about the evolution of Id1 molecule and its role in the MCLR induced cell differentiation and cancer promoting in bighead carp. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
关键词[WOS]	LOOP-HELIX PROTEINS ; CRUCIAN CARP ; DNA-BINDING ; IN-VIVO ; GENE ; EMBRYOGENESIS ; ANGIOGENESIS ; INHIBITOR ; EXTRACTS ; CANCERS
收录类别	SCI
资助信息	National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
语种	英语
WOS记录号	WOS:000313155600024
公开日期	2013-10-31
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19303]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Li, Huiying,Cai, Yan,Xie, Ping,et al. Identification and expression profile of Id1 in bighead carp in response to microcystin-LR[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2012,34(2):324-333.
APA	Li, Huiying.,Cai, Yan.,Xie, Ping.,Chen, Jun.,Hao, Le.,...&Xiong, Qian.(2012).Identification and expression profile of Id1 in bighead carp in response to microcystin-LR.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,34(2),324-333.
MLA	Li, Huiying,et al."Identification and expression profile of Id1 in bighead carp in response to microcystin-LR".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 34.2(2012):324-333.