Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice

doi:10.1016/j.ebiom.2022.104040

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice
	Zhang, Wenjia 3,5,6; Liang, Xiaolong 4; Zhu, Liang 5,6; Zhang, Xinyu 5,6; Jin, Zhengyu 5,6; De, Yang 2,3; Tian, Jie 1,3; Xue, Huadan 5,6
刊名	EBIOMEDICINE
	2022-06-01
卷号	80 页码:16
关键词	Pancreatic ductal adenocarcinoma (PDAC) Magnetic particle imaging (MPI) Superparamagnetic iron oxide nanoparticles (SPIONs) Fluorescence molecular imaging (FMI) Magnetic resonance imaging (MRI)
ISSN号	2352-3964
DOI	10.1016/j.ebiom.2022.104040
通讯作者	Jin, Zhengyu(jinzy@pumch.cn) ; De, Yang(yang.du@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Xue, Huadan(bjdanna95@hotmail.com)
英文摘要	Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy worldwide, and the precise detection is challenging currently. Magnetic particle imaging (MPI) is suitable for imaging deep and internal PDAC tumours because of its high sensitivity and unlimited imaging depth. The purpose of this study was to utilize the MPI, in combination with fluorescence molecular imaging (FMI) and magnetic resonance imaging (MRI), to advance the in vivo precise detection of PDAC xenografts. Methods The PDAC targeted plectin-1 peptide and IRDye800CW were conjugated to the superparamagnetic iron oxide nanoparticles (PTP-Fe3O4-IRDye800CW) for the PDAC-targeting triple-modality imaging. Subcutaneous and orthotopic PDAC mouse models were established. FMI, MPI, and MRI were performed for dynamic and quantitative observation of PDAC tumours. Histological staining analyses were used for ex vivo validation. Findings PTP-Fe3O4-IRDye800CW nanoparticles possessed great triple-modality imaging performance and specific targeting to plectin-1 expressed on PDAC cells. For in vivo multi-modality imaging of orthotopic PDAC models, the PTP-Fe3O4-IRDye800CW nanoparticles demonstrated higher specificity, even distribution, and longer retention effects in tumours for over 7 d compared with Con-Fe3O4-IRDye800CW nanoparticles. (MPI, 2d post-injection: PTP-Fe3O4-IRDye800CW: 85.72% +/- 1.53% vs. Con-Fe3O4-IRDye800CW: 74.41% +/- 1.91%, **P < 0.01 (Student's t test)). Ex vivo histological and Prussian blue stainings were performed to validate the distribution of probes. Interpretation These data demonstrate the feasibility of utilizing MPI for in vivo PDAC imaging and complement with FMI/MRI for a precise and comprehensive in vivo characterization of PDAC. This may benefit PDAC patients for precise diagnosis and guidance of therapy. Copyright (c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
资助项目	National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[82071896] ; National Natural Science Foundation of China[81871422] ; National Natural Science Foundation of China[81871514] ; National Natural Science Foundation of China[81227901] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401] ; Beijing Natural Science Foundation[7212207] ; Elite Program of Dong Cheng District of Beijing[2020-dchrcpyzz-28] ; Peking University Third Hospital[BYSYZD2019018] ; Peking University Third Hospital[jyzc2018-02]
WOS关键词	IRON-OXIDE NANOPARTICLES ; CANCER ; FERUMOXYTOL ; MRI
WOS研究方向	General & Internal Medicine ; Research & Experimental Medicine
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000829908400014
资助机构	National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Natural Science Foundation ; Elite Program of Dong Cheng District of Beijing ; Peking University Third Hospital
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/49752]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Jin, Zhengyu; De, Yang; Tian, Jie; Xue, Huadan
作者单位	1.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing 100191, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, State Key Lab Management & Control Complex Syst, Beijing Key Lab Mol Imaging, CAS Key Lab Mol Imaging,Inst Automat, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China 4.Peking Univ Third Hosp, Dept Ultrasound, Beijing 100191, Peoples R China 5.Chinese Acad Med Sci, 1 Shuaifuyuan, Beijing 100730, Peoples R China 6.Peking Union Med Coll, Dept Radiol, State Key Lab Complex Severe & Rare Dis, Peking Union Med Coll Hosp, 1 Shuaifuyuan, Beijing 100730, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wenjia,Liang, Xiaolong,Zhu, Liang,et al. Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice[J]. EBIOMEDICINE,2022,80:16.
APA	Zhang, Wenjia.,Liang, Xiaolong.,Zhu, Liang.,Zhang, Xinyu.,Jin, Zhengyu.,...&Xue, Huadan.(2022).Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice.EBIOMEDICINE,80,16.
MLA	Zhang, Wenjia,et al."Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice".EBIOMEDICINE 80(2022):16.