Aqueous two-phase systems evolved double-layer film for enzymatic activity preservation: A universal protein storage strategy for paper based microdevice

doi:10.1016/j.aca.2022.339540

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Aqueous two-phase systems evolved double-layer film for enzymatic activity preservation: A universal protein storage strategy for paper based microdevice
	Zhang, Qingquan 2; Chen, Jiaqu 2; Zhong, Ziyin 2; Li, Bowei 1; Gai, Hongwei 2
刊名	ANALYTICA CHIMICA ACTA
	2022-03-08
卷号	1197 页码:6
关键词	Aqueous two-phase systems Microfluidic paper-based analytical devices Protein preservation
ISSN号	0003-2670
DOI	10.1016/j.aca.2022.339540
通讯作者	Li, Bowei(bwli@yic.ac.cn) ; Gai, Hongwei(gai@jsnu.edu.cn)
英文摘要	Integration and storage of bioactive reagents is an important and challenging task in microfluidic paper-based analytical devices (mu PADs). Here, we developed a convenient and universal method to store proteins and preserve their activities in mu PADs by using aqueous two-phase systems (ATPs) evolved film. A polyethylene glycol (PEG)-dextran (DEX) double-layer film was formed through dehydration of ATPs. Functional biomolecules were stored in the bottom DEX layer on the basis of the biased partitioning and rehydrated conveniently by simple addition of buffer solution at usage. As a demonstration, enzyme immunoassay (EIA) of carcinoembryonic antigen was performed successfully on mu PAD integrated with antibodies. Even after 104 days of storage at 4 degrees C and ambient conditions, the EIA signal just lost less than 10% and 30%, which meet the storage requirements of invitro diagnosis reagents. The ATPs evolved double-layer film has double functions of stabilization and insulation, and provide a high efficiency of biomolecule preservation, thereby promoting the applications of mu PADs in POC diagnostic assay. (C) 2022 Elsevier B.V. All rights reserved.
WOS关键词	FLUID TRANSPORT ; DEVICE ; ANTIBODIES ; STABILITY ; ELISA
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000750845200002
资助机构	Natural Science Foundation of China ; Xuzhou key plan of RD
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31477]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Li, Bowei; Gai, Hongwei
作者单位	1.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Jiangsu Normal Univ, Sch Chem & Mat Sci, Jiangsu Key Lab Green Synth Funct Mat, Xuzhou 221116, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qingquan,Chen, Jiaqu,Zhong, Ziyin,et al. Aqueous two-phase systems evolved double-layer film for enzymatic activity preservation: A universal protein storage strategy for paper based microdevice[J]. ANALYTICA CHIMICA ACTA,2022,1197:6.
APA	Zhang, Qingquan,Chen, Jiaqu,Zhong, Ziyin,Li, Bowei,&Gai, Hongwei.(2022).Aqueous two-phase systems evolved double-layer film for enzymatic activity preservation: A universal protein storage strategy for paper based microdevice.ANALYTICA CHIMICA ACTA,1197,6.
MLA	Zhang, Qingquan,et al."Aqueous two-phase systems evolved double-layer film for enzymatic activity preservation: A universal protein storage strategy for paper based microdevice".ANALYTICA CHIMICA ACTA 1197(2022):6.