Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity

doi:10.1016/S2095-3119(14)60940-0

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	Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity
	Song Bing-yan ; Shi Jin-xiao ; Song Song-quan
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2015
卷号	14 期号:8 页码:1627-1636
关键词	after-ripening deep physiological dormancy Echinochloa crus-galli galactomannan-hydrolysing enzyme phytohormone stratification
ISSN号	2095-3119
DOI	10.1016/S2095-3119(14)60940-0
文献子类	Article
英文摘要	Echinochloa crus-galli, one of the world's most serious weeds, influences seriously the yield and quality of cereal crop plant. It is well known that E. crus-galli grain is dormant, but its dormant type, as well as its dormancy release and germination in relation to galactomannan-hydrolysing enzyme activity were poorly understood. The cooperation of endo-beta-mannanase (EC 3.2.1.78), beta-mannosidase (EC 3.2.1.25) and a-galactosidase (EC 3.2.1.22) can hydrolyze the cell walls rich in mannan-based polymers. In the present paper, the mature grains are used as experimental materials, we investigated the water uptake of grains, the effect of removing partial endosperm, after-ripening, stratification and phytohormone on grain germination, and the change in endo-beta-mannanase, beta-mannosidase and alpha-galactosidase activities of grains during after-ripening and germination. The results showed that the freshly-collected grains were water-permeable and had only phase I and II of water uptake, while the grains after-ripened for 150 d had an obvious phase III of water uptake. In alternating photoperiod, the germination of grains freshly-collected was zero at 10-35 degrees C, and that of half grains was 11% at 20 degrees C only. The grain germination was notably promoted by after-ripening and stratification, but not by gibberellic acid and 6-benzyladenine at 0.0001-1 mmol L-1. beta-Mannosidase activity increased during 0 to 300 d of after-ripening and then decreased. The activity of endo-beta-mannanase and alpha-galactosidase of grains decreased with after-ripening. During grain germination, endo-beta-mannanase and beta-mannosidase activities obviously increased, while alpha-galactosidase activity decreased. Our data showed that E. crus-galli grain was a deep physiological dormant, the dormancy release by after-ripening was related to an increasing beta-mannosidase activity, and its germination was closely associated with an increasing endo-beta-mannanase and beta-mannosidase activity; which have provided new knowledge to decrease the harm of E. crus-galli on production of cereal crop plant.
学科主题	Agriculture, Multidisciplinary
出版地	OXFORD
WOS关键词	ENDO-BETA-MANNANASE ; SEED-GERMINATION ; ARABIDOPSIS-THALIANA ; ABSCISIC-ACID ; TEMPERATURE ; STRATIFICATION ; SCARIFICATION ; ADAPTATION ; ENDOSPERM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000359751600018
资助机构	National Science and Technology Support Program of China [2012BAC01B05]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25644]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Jishou Univ, Coll Biol Resources & Environm Sci, Jishou 416000, Peoples R China 3.[Song Bing-yan
推荐引用方式 GB/T 7714	Song Bing-yan,Shi Jin-xiao,Song Song-quan. Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2015,14(8):1627-1636.
APA	Song Bing-yan,Shi Jin-xiao,&Song Song-quan.(2015).Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity.JOURNAL OF INTEGRATIVE AGRICULTURE,14(8),1627-1636.
MLA	Song Bing-yan,et al."Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity".JOURNAL OF INTEGRATIVE AGRICULTURE 14.8(2015):1627-1636.