A proteomic analysis of rice seed germination as affected by high temperature and ABA treatment

doi:10.1111/ppl.12292

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	A proteomic analysis of rice seed germination as affected by high temperature and ABA treatment
	Liu, Shu-Jun ; Xu, Heng-Heng ; Wang, Wei-Qing ; Li, Ni ; Wang, Wei-Ping ; Moller, Ian Max 1; Song, Song-Quan
刊名	PHYSIOLOGIA PLANTARUM
	2015
卷号	154 期号:1 页码:142-161
ISSN号	0031-9317
DOI	10.1111/ppl.12292
文献子类	Article
英文摘要	Seed germination is a critical phase in the plant life cycle, but the specific events associated with seed germination are still not fully understood. In this study, we used two-dimensional gel electrophoresis followed by mass spectrometry to investigate the changes in the proteome during imbibition of Oryza sativa seeds at optimal temperature with or without abscisic acid (ABA) and high temperature (germination thermoinhibition) to further identify and quantify key proteins required for seed germination. A total of 121 protein spots showed a significant change in abundance (1.5-fold increase/decrease) during germination under all conditions. Among these proteins, we found seven proteins specifically associated with seed germination including glycosyl hydrolases family 38 protein, granule-bound starch synthase 1, Os03g0842900 (putative steroleosin-B), N-carbamoylputrescine amidase, spermidine synthase 1, tubulin -1 chain and glutelin type-A; and a total of 20 imbibition response proteins involved in energy metabolism, cell growth, cell defense and storage proteins. High temperature inhibited seed germination by decreasing the abundance of proteins involved in methionine metabolism, amino acid biosynthesis, energy metabolism, reserve degradation, protein folding and stress responses. ABA treatment inhibited germination and decreased the abundance of proteins associated with methionine metabolism, energy production and cell division. Our results show that changes in many biological processes including energy metabolism, protein synthesis and cell defense and rescue occurred as a result of all treatments, while enzymes involved in methionine metabolism and weakening of cell wall specifically accumulated when the seeds germinated at the optimal temperature.
学科主题	Plant Sciences
电子版国际标准刊号	1399-3054
出版地	HOBOKEN
WOS关键词	UNIVERSAL STRESS-PROTEIN ; HEAT-SHOCK PROTEINS ; ABSCISIC-ACID ; EMBRYONIC PROTEINS ; MESSENGER-RNAS ; ARABIDOPSIS ; EXPRESSION ; GIBBERELLIN ; DORMANCY ; THERMOINHIBITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000353067500011
资助机构	National Science and Technology Support Program [2012BAC01B05] ; Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25618]
专题	中科院北方资源植物重点实验室
作者单位	1.State Key Lab Hybrid Rice, Grp Seed Biol, Changsha 410125, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark
推荐引用方式 GB/T 7714	Liu, Shu-Jun,Xu, Heng-Heng,Wang, Wei-Qing,et al. A proteomic analysis of rice seed germination as affected by high temperature and ABA treatment[J]. PHYSIOLOGIA PLANTARUM,2015,154(1):142-161.
APA	Liu, Shu-Jun.,Xu, Heng-Heng.,Wang, Wei-Qing.,Li, Ni.,Wang, Wei-Ping.,...&Song, Song-Quan.(2015).A proteomic analysis of rice seed germination as affected by high temperature and ABA treatment.PHYSIOLOGIA PLANTARUM,154(1),142-161.
MLA	Liu, Shu-Jun,et al."A proteomic analysis of rice seed germination as affected by high temperature and ABA treatment".PHYSIOLOGIA PLANTARUM 154.1(2015):142-161.