Glycosyltransferase-like protein ABI8/ELD1/KOB1 promotes Arabidopsis hypocotyl elongation through regulating cellulose biosynthesis

doi:10.1111/pce.12395

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	Glycosyltransferase-like protein ABI8/ELD1/KOB1 promotes Arabidopsis hypocotyl elongation through regulating cellulose biosynthesis
	Wang, Xin 1; Jing, Yanjun ; Zhang, Baocai 3,4; Zhou, Yihua 3,4; Lin, Rongcheng
刊名	PLANT CELL AND ENVIRONMENT
	2015
卷号	38 期号:3 页码:411-422
关键词	cellulose synthesis light photomorphogenesis
ISSN号	0140-7791
DOI	10.1111/pce.12395
文献子类	Article
英文摘要	Seedling de-etiolation (photomorphogenesis) is an important light-regulated developmental process in plants. Here, we showed that disruption of the gene encoding a glycosyltransferase-like protein, ABA INSENSITIVE 8 (ABI8)/ELONGATION EFFECTIVE 1 (ELD1)/KOBITO1 (KOB1), caused short-hypocotyl elongation under all light conditions examined and even in darkness. We found that the ABI8 transcript level was down-regulated by light in a phytochrome A-dependent manner. Furthermore, light destabilized ABI8 protein via the 26S proteasome degradation pathway. We showed that ABI8 promoted the expression of genes involved in cell elongation and cellulose synthesis. Consistently, the cellulose content was reduced in the abi8 mutants and application of 2, 6-dichlorobenzonitrile (an inhibitor of cellulose biosynthesis) mimicked the abi8 mutant phenotype. Moreover, we found that phytochrome and cryptochrome photoreceptors negatively, whereas CONSTITUTIVE PHOTOMORPHOGENIC 1 positively, regulated cellulose synthesis. We also showed that ELONGATED HYPOCOTYL 5 directly bound to the promoters of ABI8 and several cellulose synthesis genes and repressed their expression in light conditions. Taken together, our study reveals that ABI8 functions as a negative factor in light inhibition of hypocotyl elongation through modulating cellulose biosynthesis. This study elucidates a molecular mechanism on how light regulates cellulose synthesis and hypocotyl elongation during plant photomorphogenic growth. The authors demonstrate that ABI8 is a negative regulator of light-inhibition of hypocotyl growth and that HY5 transcription factor directly represses ABI8 expression. ABI8 are down-regulated by light both at the transcription and post-translational levels.
学科主题	Plant Sciences
电子版国际标准刊号	1365-3040
出版地	HOBOKEN
WOS关键词	TRANSCRIPTION FACTOR HY5 ; ABSCISIC-ACID ; PHYTOCHROME-B ; CELL ELONGATION ; INTEGRATE LIGHT ; GENE ; PHOTOMORPHOGENESIS ; ENCODES ; COP1 ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000349994600004
资助机构	National Natural Science Foundation of China [31170221, 31325002, 31125019] ; Ministry of Agriculture of China [2011ZX08009-003]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25800]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xin,Jing, Yanjun,Zhang, Baocai,et al. Glycosyltransferase-like protein ABI8/ELD1/KOB1 promotes Arabidopsis hypocotyl elongation through regulating cellulose biosynthesis[J]. PLANT CELL AND ENVIRONMENT,2015,38(3):411-422.
APA	Wang, Xin,Jing, Yanjun,Zhang, Baocai,Zhou, Yihua,&Lin, Rongcheng.(2015).Glycosyltransferase-like protein ABI8/ELD1/KOB1 promotes Arabidopsis hypocotyl elongation through regulating cellulose biosynthesis.PLANT CELL AND ENVIRONMENT,38(3),411-422.
MLA	Wang, Xin,et al."Glycosyltransferase-like protein ABI8/ELD1/KOB1 promotes Arabidopsis hypocotyl elongation through regulating cellulose biosynthesis".PLANT CELL AND ENVIRONMENT 38.3(2015):411-422.