Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes

doi:10.1016/j.bbabio.2014.11.005

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes
	Sun, Ruixue 2; Liu, Kun ; Dong, Lianqing 2; Wu, Yuling 2; Paulsen, Harald 1; Yang, Chunhong
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
	2015
卷号	1847 期号:2 页码:248-261
关键词	Proteoliposome Protein-protein interaction Photosystem II Light-harvesting complex Minor antenna
ISSN号	0005-2728
DOI	10.1016/j.bbabio.2014.11.005
文献子类	Article
英文摘要	Minor antennae of photosystem (PS) II, located between the PSII core complex and the major antenna (LHCII), are important components for the structural and functional integrity of PSII supercomplexes. In order to study the functional significance of minor antennae in the energetic coupling between LHCII and the PSII core, characteristics of PSII-LHCII proteoliposomes, with or without minor antennae, were investigated. Two types of PSII preparations containing different antenna compositions were isolated from pea: 1) the PSII preparation composed of the PSII core complex, all of the minor antennae, and a small amount of major antennae (MCC); and 2) the purified PSII dimeric core complexes without periphery antenna (CC). They were incorporated, together with LHCII, into liposomes composed of thylakoid membrane lipids. The spectroscopic and functional characteristics were measured. 77 K fluorescence emission spectra revealed an increased spectral weight of fluorescence from PSII reaction center in the CC-LHCII proteoliposomes, implying energetic coupling between LHCII and CC in the koteoliposomes lacking minor antennae. This result was further confirmed by chlorophyll a fluorescence induction kinetics. The incorporation of LHCII together with CC markedly increased the antenna cross-section of the PSII core complex. The 2,6-dichlorophenolindophenol photoreduction measurement implied that the lack of minor antennae in PSII supercomplexes did not block the energy transfer from LHCII to the PSII core complex. In conclusion, it is possible, in liposomes, that LHCII transfer energy directly to the PSII core complex, in the absence of minor antennae. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
电子版国际标准刊号	0006-3002
出版地	AMSTERDAM
WOS关键词	LIGHT-HARVESTING COMPLEX ; CHLOROPHYLL-PROTEIN COMPLEXES ; TANDEM MASS-SPECTROMETRY ; SUPRAMOLECULAR ORGANIZATION ; CRYSTAL-STRUCTURE ; ARABIDOPSIS-THALIANA ; THYLAKOID MEMBRANES ; ANGSTROM RESOLUTION ; HIGHER-PLANTS ; CHLAMYDOMONAS-REINHARDTII
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000348337400012
资助机构	National Basic Research Program of China [2011CBA00904] ; Knowledge Innovation Program of the Chinese Academy of Sciences [KSZD-EW-Z-018] ; National Natural Science Foundation of China [31070212, 31370275] ; Deutsche Forschungsgemeinschaft [SFB 625-TP B7]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25718]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Nanxincun 20, Beijing 100093, Peoples R China 3.Johannes Gutenberg Univ Mainz, Inst Allgemeine Bot, D-55099 Mainz, Germany
推荐引用方式 GB/T 7714	Sun, Ruixue,Liu, Kun,Dong, Lianqing,et al. Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2015,1847(2):248-261.
APA	Sun, Ruixue,Liu, Kun,Dong, Lianqing,Wu, Yuling,Paulsen, Harald,&Yang, Chunhong.(2015).Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1847(2),248-261.
MLA	Sun, Ruixue,et al."Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1847.2(2015):248-261.