High-throughput deep sequencing reveals that microRNAs play important roles in salt tolerance of euhalophyte Salicornia europaea

doi:10.1186/s12870-015-0451-3

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	High-throughput deep sequencing reveals that microRNAs play important roles in salt tolerance of euhalophyte Salicornia europaea
	Feng, Juanjuan ; Wang, Jinhui ; Fan, Pengxiang 4; Jia, Weitao ; Nie, Lingling ; Jiang, Ping ; Chen, Xianyang ; Lv, Sulian ; Wan, Lichuan ; Chang, Sandra 1,3
刊名	BMC PLANT BIOLOGY
	2015
卷号	15
关键词	Salicornia europaea Euhalophyte miRNA Deep sequencing Salt stress Lignin biosynthesis Biofuel crop
ISSN号	1471-2229
DOI	10.1186/s12870-015-0451-3
文献子类	Article
英文摘要	Background: microRNAs (miRNAs) are implicated in plant development processes and play pivotal roles in plant adaptation to environmental stresses. Salicornia europaea, a salt mash euhalophyte, is a suitable model plant to study salt adaptation mechanisms. S. europaea is also a vegetable, forage, and oilseed that can be used for saline land reclamation and biofuel precursor production on marginal lands. Despite its importance, no miRNA has been identified from S. europaea thus far. Results: Deep sequencing was performed to investigate small RNA transcriptome of S. europaea. Two hundred and ten conserved miRNAs comprising 51 families and 31 novel miRNAs (including seven miRNA star sequences) belonging to 30 families were identified. About half (13 out of 31) of the novel miRNAs were only detected in salt-treated samples. The expression of 43 conserved and 13 novel miRNAs significantly changed in response to salinity. In addition, 53 conserved and 13 novel miRNAs were differentially expressed between the shoots and roots. Furthermore, 306 and 195 S. europaea unigenes were predicted to be targets of 41 conserved and 29 novel miRNA families, respectively. These targets encoded a wide range of proteins, and genes involved in transcription regulation constituted the largest category. Four of these genes encoding laccase, F-box family protein, SAC3/GANP family protein, and NADPH cytochrome P-450 reductase were validated using 5'-RACE. Conclusions: Our results indicate that specific miRNAs are tightly regulated by salinity in the shoots and/or roots of S. europaea, which may play important roles in salt tolerance of this euhalophyte. The S. europaea salt-responsive miRNAs and miRNAs that target transcription factors, nucleotide binding site-leucine-rich repeat proteins and enzymes involved in lignin biosynthesis as well as carbon and nitrogen metabolism may be applied in genetic engineering of crops with high stress tolerance, and genetic modification of biofuel crops with high biomass and regulatable lignin biosynthesis.
学科主题	Plant Sciences
出版地	LONDON
WOS关键词	STRESS-REGULATED MICRORNAS ; COMPARATIVE PROTEOMIC ANALYSIS ; SMALL RNAS ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; DIFFERENTIAL EXPRESSION ; WIDE IDENTIFICATION ; GLYCOLYTIC ENZYME ; DOWN-REGULATION ; HIGH-SALINITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	BMC
WOS记录号	WOS:000350380000001
资助机构	Research Programs from the Chinese Ministry of Agriculture [2014ZX08009-003-002] ; International Scientific and Technological Cooperation Project [2012DFG61720]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25939]
专题	中科院植物分子生理学重点实验室
作者单位	1.Tsinghua Univ, Beijing Engn Res Ctr Biofuels, Beijing 100084, Peoples R China 2.Tsinghua Univ, Inst Nucl & New Energy Technol, Beijing 100084, Peoples R China 3.Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA 4.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Feng, Juanjuan,Wang, Jinhui,Fan, Pengxiang,et al. High-throughput deep sequencing reveals that microRNAs play important roles in salt tolerance of euhalophyte Salicornia europaea[J]. BMC PLANT BIOLOGY,2015,15.
APA	Feng, Juanjuan.,Wang, Jinhui.,Fan, Pengxiang.,Jia, Weitao.,Nie, Lingling.,...&Li, Yinxin.(2015).High-throughput deep sequencing reveals that microRNAs play important roles in salt tolerance of euhalophyte Salicornia europaea.BMC PLANT BIOLOGY,15.
MLA	Feng, Juanjuan,et al."High-throughput deep sequencing reveals that microRNAs play important roles in salt tolerance of euhalophyte Salicornia europaea".BMC PLANT BIOLOGY 15(2015).