Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth

doi:10.1074/mcp.M114.047225

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth
	Suo, Jinwei ; Zhao, Qi ; Zhang, Zhengxiu ; Chen, Sixue 2; Cao, Jian'guo ; Liu, Guanjun 1; Wei, Xing 1; Wang, Tai 4; Yang, Chuanping 1; Dai, Shaojun 1
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2015
卷号	14 期号:9 页码:2510-2534
ISSN号	1535-9476
DOI	10.1074/mcp.M114.047225
文献子类	Article
英文摘要	Fern spore is a good single-cell model for studying the sophisticated molecular networks in asymmetric cell division, differentiation, and polar growth. Osmunda cinnamomea L. var. asiatica is one of the oldest fern species with typical separate-growing trophophyll and sporophyll. The chlorophyllous spores generated from sporophyll can germinate without dormancy. In this study, the spore ultrastructure, antioxidant enzyme activities, as well as protein and gene expression patterns were analyzed in the course of spore germination at five typical stages (i.e. mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Proteomic analysis revealed 113 differentially expressed proteins, which were mainly involved in photosynthesis, reserve mobilization, energy supplying, protein synthesis and turnover, reactive oxygen species scavenging, signaling, and cell structure modulation. The presence of multiple proteoforms of 25 differentially expressed proteins implies that post-translational modification may play important roles in spore germination. The dynamic patterns of proteins and their encoding genes exhibited specific characteristics in the processes of cell division and rhizoid tip growth, which include heterotrophic and autotrophic metabolisms, de novo protein synthesis and active protein turnover, reactive oxygen species and hormone (brassinosteroid and ethylene) signaling, and vesicle trafficking and cytoskeleton dynamic. In addition, the function skew of proteins in fern spores highlights the unique and common mechanisms when compared with evolutionarily divergent spermatophyte pollen. These findings provide an improved understanding of the typical single-celled asymmetric division and polar growth during fern spore germination.
学科主题	Biochemical Research Methods
电子版国际标准刊号	1535-9484
出版地	BETHESDA
WOS关键词	POLLEN-TUBE GROWTH ; DIFFERENTIALLY EXPRESSED PROTEINS ; ARABIDOPSIS SEED-GERMINATION ; STORED MESSENGER-RNA ; CERATOPTERIS-RICHARDII ; PROMOTES GERMINATION ; ONOCLEA-SENSIBILIS ; CALCIUM CURRENT ; MATURE POLLEN ; NITRIC-OXIDE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000360823000017
资助机构	National Natural Science Foundation of China [31071194, 31270310] ; Fundamental Research Funds for the Central Universities [2572014EA04] ; Postdoctoral Science Foundation of China [201104407] ; Funds for Distinguished Young Scientists of Heilongjiang Province [JC201011] ; Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China [40-NEFU-01]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25720]
专题	中科院植物分子生理学重点实验室
作者单位	1.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Harbin 150040, Peoples R China 2.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China 3.Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China 4.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Dept Biol, Genet Inst,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA 5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Suo, Jinwei,Zhao, Qi,Zhang, Zhengxiu,et al. Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(9):2510-2534.
APA	Suo, Jinwei.,Zhao, Qi.,Zhang, Zhengxiu.,Chen, Sixue.,Cao, Jian'guo.,...&Dai, Shaojun.(2015).Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth.MOLECULAR & CELLULAR PROTEOMICS,14(9),2510-2534.
MLA	Suo, Jinwei,et al."Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth".MOLECULAR & CELLULAR PROTEOMICS 14.9(2015):2510-2534.