Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing

doi:10.1186/s12870-015-0530-5

CORC > 植物研究所 > 中国科学院植物研究所 > 植被与环境变化国家重点实验室

	Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing
	Wang, Tian-Zuo ; Liu, Min ; Zhao, Min-Gui ; Chen, Rujin 1; Zhang, Wen-Hao
刊名	BMC PLANT BIOLOGY
	2015
卷号	15
关键词	Long non-coding RNAs (lncRNAs) Osmotic stress Salt stress Medicago truncatula Legume plants High-throughput sequencing Transcriptional regulation
ISSN号	1471-2229
DOI	10.1186/s12870-015-0530-5
文献子类	Article
英文摘要	Background: Long non-coding RNAs (lncRNAs) have been shown to play crucially regulatory roles in diverse biological processes involving complex mechanisms. However, information regarding the number, sequences, characteristics and potential functions of lncRNAs in plants is so far overly limited. Results: Using high-throughput sequencing and bioinformatics analysis, we identified a total of 23,324 putative lncRNAs from control, osmotic stress-and salt stress-treated leaf and root samples of Medicago truncatula, a model legume species. Out of these lncRNAs, 7,863 and 5,561 lncRNAs were identified from osmotic stress-treated leaf and root samples, respectively. While, 7,361 and 7,874 lncRNAs were identified from salt stress-treated leaf and root samples, respectively. To reveal their potential functions, we analyzed Gene Ontology (GO) terms of genes that overlap with or are neighbors of the stress-responsive lncRNAs. Enrichments in GO terms in biological processes such as signal transduction, energy synthesis, molecule metabolism, detoxification, transcription and translation were found. Conclusions: LncRNAs are likely involved in regulating plant's responses and adaptation to osmotic and salt stresses in complex regulatory networks with protein-coding genes. These findings are of importance for our understanding of the potential roles of lncRNAs in responses of plants in general and M. truncatula in particular to abiotic stresses.
学科主题	Plant Sciences
出版地	LONDON
WOS关键词	NATURAL ANTISENSE TRANSCRIPTS ; ARABIDOPSIS-THALIANA ; SIGNAL-TRANSDUCTION ; GENE-EXPRESSION ; MESSENGER-RNA ; DROUGHT ; PREDICTION ; TOLERANCE ; MICRORNAS ; EVOLUTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000355968400001
资助机构	National Natural Science Foundation of China [31300231, 31272234] ; National Basic Research Program of China [2015CB150800] ; External Cooperation Program of the Chinese Academy of Sciences [151111KYSB20130008]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25983]
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Global Change Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, State Key Lab Vegetat & Environm Change, Inst Bot, Beijing 100093, Peoples R China 3.Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA
推荐引用方式 GB/T 7714	Wang, Tian-Zuo,Liu, Min,Zhao, Min-Gui,et al. Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing[J]. BMC PLANT BIOLOGY,2015,15.
APA	Wang, Tian-Zuo,Liu, Min,Zhao, Min-Gui,Chen, Rujin,&Zhang, Wen-Hao.(2015).Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing.BMC PLANT BIOLOGY,15.
MLA	Wang, Tian-Zuo,et al."Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing".BMC PLANT BIOLOGY 15(2015).