Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia

doi:10.1093/jxb/erv365

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	Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia
	Du, Hui ; Wu, Jie 4; Ji, Kui-Xian ; Zeng, Qing-Yin ; Bhuiya, Mohammad-Wadud ; Su, Shang 4; Shu, Qing-Yan ; Ren, Hong-Xu ; Liu, Zheng-An ; Wang, Liang-Sheng
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2015
卷号	66 期号:21 页码:6563-6577
关键词	Anthocyanin O-methyltransferase catalytic activity flavonoids flower coloration Paeonia single amino acid substitution
ISSN号	0022-0957
DOI	10.1093/jxb/erv365
文献子类	Article
英文摘要	Anthocyanins are major pigments in plants. Methylation plays a role in the diversity and stability of anthocyanins. However, the contribution of anthocyanin methylation to flower coloration is still unclear. We identified two homologous anthocyanin O-methyltransferase (AOMT) genes from purple-flowered (PsAOMT) and red-flowered (PtAOMT) Paeonia plants, and we performed functional analyses of the two genes in vitro and in vivo. The critical amino acids for AOMT catalytic activity were studied by site-directed mutagenesis. We showed that the recombinant proteins, PsAOMT and PtAOMT, had identical substrate preferences towards anthocyanins. The methylation activity of PsAOMT was 60 times higher than that of PtAOMT in vitro. Interestingly, this vast difference in catalytic activity appeared to result from a single amino acid residue substitution at position 87 (arginine to leucine). There were significant differences between the 35S::PsAOMT transgenic tobacco and control flowers in relation to their chromatic parameters, which further confirmed the function of PsAOMT in vivo. The expression levels of the two homologous AOMT genes were consistent with anthocyanin accumulation in petals. We conclude that AOMTs are responsible for the methylation of cyanidin glycosides in Paeonia plants and play an important role in purple coloration in Paeonia spp.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	PETAL ANTHOCYANINS ; PROTEIN-STRUCTURE ; GENETIC-CONTROL ; CYCLAMEN SPP. ; COPIGMENTATION ; EVOLUTION ; BIOSYNTHESIS ; PIGMENT ; TOOL ; IDENTIFICATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000367814500004
资助机构	National Natural Science Foundation of China [30771521, 31071824] ; National High Technology Research and Development Program of China (863 program) [2011AA10020702]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25868]
专题	系统与进化植物学国家重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.MOgene Green Chem, St Louis, MO 63132 USA 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Du, Hui,Wu, Jie,Ji, Kui-Xian,et al. Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(21):6563-6577.
APA	Du, Hui.,Wu, Jie.,Ji, Kui-Xian.,Zeng, Qing-Yin.,Bhuiya, Mohammad-Wadud.,...&Wang, Liang-Sheng.(2015).Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia.JOURNAL OF EXPERIMENTAL BOTANY,66(21),6563-6577.
MLA	Du, Hui,et al."Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia".JOURNAL OF EXPERIMENTAL BOTANY 66.21(2015):6563-6577.