Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation

doi:10.1093/jxb/erv173

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	Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation
	Zhu, Zhixin 2; Wang, Hailong 2; Wang, Yiting 2; Guan, Shan ; Wang, Fang 2; Tang, Jingyu 2; Zhang, Ruijuan 2; Xie, Lulu 2; Lu, Yingqing 2
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2015
卷号	66 期号:13 页码:3775-3789
关键词	Anthocyanin pathway Arabidopsis bHLH cis element Ipomoea MBW complex MYB promoter activity promoter architecture regulatory module trans factor transcription initiation WDR
ISSN号	0022-0957
DOI	10.1093/jxb/erv173
文献子类	Article
英文摘要	Cellular activities such as compound synthesis often require the transcriptional activation of an entire pathway; however, the molecular mechanisms underlying pathway activation have rarely been explained. Here, the cis regulatory architecture of the anthocyanin pathway genes targeted by the transcription factor (TF) complex including MYB, bHLH, and WDR was systematically analysed in one species and the findings extended to others. In Ipomoea purpurea, the IpMYB1-IpbHLH2-IpWDR1 (IpMBW) complex was found to be orthologous to the PAP1-GL3-TTG1 (AtPGT) complex of Arabidopsis thaliana, and interacted with a 7-bp MYB-recognizing element (MRE) and a 6-bp bHLH-recognizing element (BRE) at the proximal promoter region of the pathway genes. There was little transcription of the gene in the absence of the MRE or BRE. The cis elements identified experimentally converged on two syntaxes, ANCNNCC for MREs and CACN(A/C/T)(G/T) for BREs, and our bioinformatic analysis showed that these were present within anthocyanin gene promoters in at least 35 species, including both gymnosperms and angiosperms. For the anthocyanin pathway, IpMBW and AtPGT recognized the interspecific promoters of both early and later genes. In A. thaliana, the seed-specific TF complex (TT2, TT8, and TTG1) may regulate all the anthocyanin pathway genes, in addition to the proanthocyanidin-specific BAN. When multiple TF complexes in the anthocyanin pathway were compared, the cis architecture played a role larger than the TF complex in determining the variation in promoter activity. Collectively, a cis logic common to the pathway gene promoters was found, and this logic is essential for the trans factors to regulate the pathway.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	CHALCONE SYNTHASE PROMOTER ; ARABIDOPSIS-THALIANA ; MORNING GLORY ; TRANSCRIPTION FACTORS ; REPEAT PROTEIN ; MYB DOMAIN ; PHENYLPROPANOID BIOSYNTHESIS ; EXPRESSION PATTERNS ; FLAVONOID SYNTHESIS ; IPOMOEA-PURPUREA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000358533400010
资助机构	National Science Foundation of China [91331116, 31070263] ; Chinese Academy of Sciences [KSCX2-YW-N-043]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25790]
专题	系统与进化植物学国家重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Zhixin,Wang, Hailong,Wang, Yiting,et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(13):3775-3789.
APA	Zhu, Zhixin.,Wang, Hailong.,Wang, Yiting.,Guan, Shan.,Wang, Fang.,...&Lu, Yingqing.(2015).Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.JOURNAL OF EXPERIMENTAL BOTANY,66(13),3775-3789.
MLA	Zhu, Zhixin,et al."Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation".JOURNAL OF EXPERIMENTAL BOTANY 66.13(2015):3775-3789.