Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis

doi:10.1038/s41420-022-01091-5

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	Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis
	Zhao, Yan 1,2,3; Sun, Li 1,2,3,4
刊名	CELL DEATH DISCOVERY
	2022-07-04
卷号	8 期号:1 页码:8
DOI	10.1038/s41420-022-01091-5
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	Bacillus cereus is well known as a causative agent of foodborne gastrointestinal diseases and systemic non-gastrointestinal diseases. We have recently identified a pathogenic B. cereus (named H2) from a deep-sea cold-seep. H2 possesses the pyroptosis-inducing capacity and contains a number of enterotoxins including cytotoxin K (CytK). In the present work, we examined the cytotoxicity of the CytK of H2 to human macrophages. CytK bound macrophages by interaction with the plasma membrane and caused cellular structure damage. CytK-cell interaction triggered rapid pyroptosis mediated by caspase 1-activated gasdermin D (GSDMD). CytK-induced pyroptosis required NLRP3 inflammasome activation, K+ efflux, and intracellular Ca2+ accumulation. CytK exhibited apparent binding to several cytomembrane lipids, in particular phosphatidic acid, which proved to be essential to CytK-elicited cell death. Together, these results add new insights into the cytotoxic mechanism of CytK.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; CAS Key Laboratory of Molecular Virology Immunology[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province ; Institut Pasteur of Shanghai ; Chinese Academy of Sciences
WOS研究方向	Cell Biology
语种	英语
出版者	SPRINGERNATURE
WOS记录号	WOS:000820647400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179613]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yan,Sun, Li. Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis[J]. CELL DEATH DISCOVERY,2022,8(1):8.
APA	Zhao, Yan,&Sun, Li.(2022).Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis.CELL DEATH DISCOVERY,8(1),8.
MLA	Zhao, Yan,et al."Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis".CELL DEATH DISCOVERY 8.1(2022):8.