The Osmoprotectant Switch of Potassium to Compatible Solutes in an Extremely Halophilic Archaea Halorubrum kocurii 2020YC7

doi:10.3390/genes13060939

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	The Osmoprotectant Switch of Potassium to Compatible Solutes in an Extremely Halophilic Archaea Halorubrum kocurii 2020YC7
	Ding, Runting 1,2; Yang, Na 1; Liu, Jianguo 1,3
刊名	GENES
	2022-06-01
卷号	13 期号:6 页码:15
关键词	halophilic archaea Halorubrum kocurii 2020YC7 osmoprotectant strategy potassium glycine betaine trehalose
DOI	10.3390/genes13060939
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
英文摘要	The main osmoadaptive mechanisms of extremely halophilic archaea include the "salt-in" strategy and the "compatible solutes" strategy. Here we report the osmoadaptive mechanism of an extremely halophilic archaea H. kocurii 2020YC7, isolated from a high salt environment sample. Genomic data revealed that strain 2020YC7 harbors genes trkA, trkH, kch for K+ uptake, kefB for K+ output, treS for trehalose production from polysaccharide, and betaine/carnitine/choline transporter family gene for glycine betaine uptake. Strain 2020YC7 could accumulate 8.17 to 28.67 mu mol/mg protein K+ in a defined medium, with its content increasing along with the increasing salinity from 100 to 200 g/L. When exogenous glycine betaine was added, glycine betaine functioned as the primary osmotic solute between 200 and 250 g/L NaCl, which was accumulated up to 15.27 mg/mg protein in 2020YC7 cells. RT-qPCR results completely confirmed these results. Notably, the concentrations of intracellular trehalose decreased from 5.26 to 2.61 mg/mg protein as the NaCl increased from 50 to 250 g/L. In combination with this result, the transcript level of gene treS, which catalyzes the production of trehalose from polysaccharide, was significantly up-regulated at 50-100 g/L NaCl. Therefore, trehalose does not act as an osmotic solute at high NaCl concentrations (more than 100 g/L) but at relatively low NaCl concentrations (50-100 g/L). And we propose that the degradation of cell wall polysaccharide, as a source of trehalose in a low-salt environment, may be one of the reasons for the obligate halophilic characteristics of strain 2020YC7.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000] ; Young Scientists Fund of LMBB, Pilot National Laboratory for Marine Science and Technology (Qingdao), China[YQ2018NO04]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	MDPI
WOS记录号	WOS:000818263700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179610]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, CAS & Shandong Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Ding, Runting,Yang, Na,Liu, Jianguo. The Osmoprotectant Switch of Potassium to Compatible Solutes in an Extremely Halophilic Archaea Halorubrum kocurii 2020YC7[J]. GENES,2022,13(6):15.
APA	Ding, Runting,Yang, Na,&Liu, Jianguo.(2022).The Osmoprotectant Switch of Potassium to Compatible Solutes in an Extremely Halophilic Archaea Halorubrum kocurii 2020YC7.GENES,13(6),15.
MLA	Ding, Runting,et al."The Osmoprotectant Switch of Potassium to Compatible Solutes in an Extremely Halophilic Archaea Halorubrum kocurii 2020YC7".GENES 13.6(2022):15.