A near-infrared fluorescent probe was used to evaluate the role of histone deacetylase in pulmonary fibrosis cells and mice

doi:10.1016/j.snb.2022.132012

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A near-infrared fluorescent probe was used to evaluate the role of histone deacetylase in pulmonary fibrosis cells and mice
	Wei, Yinghui 1,4; Hou, Junjun 1,4; Lu, Pengpeng 2,3; Fu, Lili 2,3; Wang, Xiaoyan 2,3; Huang, Yan 2,3; Chen, Lingxin 2,3; Lv, Changjun 1,4
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2022-09-01
卷号	366 页码:8
关键词	Fluorescent probe Near-infrared Histone deacetylases Idiopathic pulmonary fibrosis Fluorescence imaging
DOI	10.1016/j.snb.2022.132012
通讯作者	Huang, Yan(yhuang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn) ; Lv, Changjun(lucky_lcj@sina.com)
英文摘要	Idiopathic pulmonary fibrosis (IPF) is a common and serious pulmonary fibrosis disease, the pathogenesis of IPF is closely related to a variety of biological small molecules and proteins, Histone deacetylases (HDACs) play an important role in inhibiting apoptosis and promoting pulmonary fibrosis. Here, we propose Cy-HDAC, (a HDACs fluorescent probe), which is selective and sensitive to HDACs and can be used to detect real-time fluctuations in HDACs activity in cells and mouse models. Our results suggest that HDAC inhibitors suberoylanilide hydroxamic acid (SAHA) treatment in the model group of pulmonary fibrosis leads to enhanced acetylation of histone H3, which allows increased transcription of some apoptosis-related proteins, promoting the activation of apoptotic protein Bak and mitochondria intrinsic apoptotic pathways, while the reduction of anti-apoptotic protein Bcl-xL promotes fibroblast apoptosis. In the bleomycin-induced pulmonary fibrosis mouse model, we found that pul-monary fibrosis severity was reduced in the SAHA-treated group, and SAHA combined with pirfenidone was superior to pirfenidone alone. Therefore, HDACs levels in IPF may provide a new detection possibility for IPF diagnosis, and HDACs may provide a novel alternative for the treatment of IPF.
WOS关键词	AGGREGATION ; PIRFENIDONE ; INHIBITORS ; NINTEDANIB ; APOPTOSIS ; DESIGN
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000803705100004
资助机构	National Nature Science Foundation of China ; Sci-ence and Technology Innovation Development Plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31156]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者	Huang, Yan; Chen, Lingxin; Lv, Changjun
作者单位	1.Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China
推荐引用方式 GB/T 7714	Wei, Yinghui,Hou, Junjun,Lu, Pengpeng,et al. A near-infrared fluorescent probe was used to evaluate the role of histone deacetylase in pulmonary fibrosis cells and mice[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,366:8.
APA	Wei, Yinghui.,Hou, Junjun.,Lu, Pengpeng.,Fu, Lili.,Wang, Xiaoyan.,...&Lv, Changjun.(2022).A near-infrared fluorescent probe was used to evaluate the role of histone deacetylase in pulmonary fibrosis cells and mice.SENSORS AND ACTUATORS B-CHEMICAL,366,8.
MLA	Wei, Yinghui,et al."A near-infrared fluorescent probe was used to evaluate the role of histone deacetylase in pulmonary fibrosis cells and mice".SENSORS AND ACTUATORS B-CHEMICAL 366(2022):8.