Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection

doi:10.1016/j.bios.2022.114282

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	Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection
	Wei, Yangdao 2; Tao, Zhenzhen 2; Wan, Lu 2; Zong, Chengli 2; Wu, Jiajia 1; Tan, Xiao 3; Wang, Buhua 2; Guo, Zixuan 2; Zhang, Ling 2; Yuan, Haoyu 2
刊名	BIOSENSORS & BIOELECTRONICS
	2022-09-01
卷号	211 页码:6
关键词	CRISPR-Cas Bacteria diagnostic Staphylococcus aureus
ISSN号	0956-5663
DOI	10.1016/j.bios.2022.114282
通讯作者	Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Wan, Yi(993602@hainanu.edu.cn)
英文摘要	CRISPR-Cas systems have been employed to detect a large variety of pathogenic microorganisms by simply changing the guide RNA sequence. However, these platforms usually rely on nucleic acid extraction and amplification to achieve good sensitivity. Herein, we developed a new platform for the highly specific and sensitive detection of live staphylococcus aureus (S. aureus) based on an Aptamer-based Cas14a1 Biosensor (ACasB), without the need for nucleic acid extraction or amplification. First, the S. aureus specific aptamer was hybrid with a blocker DNA. After the live S. aureus was added, the blocker can be released upon bacteria-aptamer binding. Finally, the released blocker can activate Cas14a1 protein by binding with the sgRNA to generate a change of fluorescent intensity. The ACasB indicates high specificity and sensitivity: it can directly distinguish 400 CFU/ml live S. aureus cells. Comparable to qPCR, the Cas14a1-aptamer biosensor can detect S. aureus with 100% accuracy in complex samples. Therefore, this ACasB for the on-site detection of live S. aureus can broaden its applications in food safety and environmental monitoring.
资助项目	Hainan Provincial Natural Science Foundation of China, China[2019RC092] ; Hainan Provincial Natural Science Foundation of China, China[420RC518] ; Hainan Provincial Natural Science Foundation of China, China[821CXTD1011] ; National Natural Science Foundation of China, China[41866002] ; National Natural Science Foundation of China, China[4216060011] ; National Natural Science Foundation of China, China[82073697] ; Open Fund of Shandong Key Laboratory of Corrosion Science, China[KLCS201910] ; Research Foundation of Hainan University, China[KYQD (ZR) 1711] ; Research Foundation of Hainan University, China[KYQD (ZR) 1997] ; Research Foundation of Hainan University, China[KYQD (ZR) 20087] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology, China[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Technology, China[JYG 2002]
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000806344000003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179437]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Yang, Zhiqing; Wan, Yi
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Hainan Univ, Sch Pharmaceut Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 3.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Wei, Yangdao,Tao, Zhenzhen,Wan, Lu,et al. Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection[J]. BIOSENSORS & BIOELECTRONICS,2022,211:6.
APA	Wei, Yangdao.,Tao, Zhenzhen.,Wan, Lu.,Zong, Chengli.,Wu, Jiajia.,...&Wan, Yi.(2022).Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection.BIOSENSORS & BIOELECTRONICS,211,6.
MLA	Wei, Yangdao,et al."Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection".BIOSENSORS & BIOELECTRONICS 211(2022):6.