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Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench
Wang, Yujian1,2,3,5; Zhang, Jian4; Yuan, Zihao1,5; Sun, Li1,2,3,5
KeywordBacillus cereus deep sea pathogenicity cytotoxicity spore
Corresponding AuthorYuan, Zihao( ; Sun, Li(
English AbstractBacillus cereus is an important opportunistic pathogen widely distributed in the environment. In this study, we reported the isolation and characterization of a B. cereus isolate, MB1, from the Challenger Deep of the Mariana Trench. MB1 is aerobic, motile, and able to form endospores. It possesses 5966 genes distributed on a circular chromosome and two plasmids. The MB1 genome contains 14 sets of 23S, 5S, and 16S ribosomal RNA operons, 106 tRNA genes, 4 sRNA genes, 12 genomic islands, 13 prophages, and 302 putative virulence genes, including enterotoxins and cytolysins. Infection studies showed that MB1 was able to cause acute and lethal infection in fish and mice, and was highly toxic to mammalian cells. MB1 induced, in a dose-dependent manner, pyroptotic cell death, characterized by activation of caspase-1, cleavage of gasdermin D, and release of IL-1 beta and IL-18. MB1 spores exhibited swimming and haemolytic capacity, but were severely attenuated in pathogenicity, which, however, was regained to the full extent when the spores germinated under suitable conditions. Taken together, these results provide new insights into the biological and pathogenic mechanism of deep sea B. cereus.
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA22050402] ; CAS Key Laboratory of Molecular Virology Immunology[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province ; Institut Pasteur of Shanghai, Chinese Academy of Sciences
WOS Research AreaImmunology ; Infectious Diseases ; Microbiology
WOS IDWOS:000814954900001
Content Type期刊论文
Corresponding AuthorYuan, Zihao; Sun, Li
Affiliation1.Chinese Acad Sci, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
4.Yan Tai Univ, Sch Ocean, Yantai, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yujian,Zhang, Jian,Yuan, Zihao,et al. Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench[J]. VIRULENCE,2022,13(1):1062-1075.
APA Wang, Yujian,Zhang, Jian,Yuan, Zihao,&Sun, Li.(2022).Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench.VIRULENCE,13(1),1062-1075.
MLA Wang, Yujian,et al."Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench".VIRULENCE 13.1(2022):1062-1075.
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