An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity

doi:10.1371/journal.pgen.1006016

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	An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity
	Lin, Xiao-Li ; Niu, De ; Hu, Zi-Liang ; Kim, Dae Heon 2; Jin, Yin Hua ; Cai, Bin ; Liu, Peng 6; Miura, Kenji 3; Yun, Dae-Jin ; Kim, Woe-Yeon
刊名	PLOS GENETICS
	2016
卷号	12 期号:4
ISSN号	1553-7404
DOI	10.1371/journal.pgen.1006016
文献子类	Article
英文摘要	COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), a ubiquitin E3 ligase, is a central negative regulator of photomorphogenesis. However, how COP1 activity is regulated by post-translational modifications remains largely unknown. Here we show that SUMO (small ubiquitin-like modifier) modification enhances COP1 activity. Loss-of-function siz1 mutant seedlings exhibit a weak constitutive photomorphogenic phenotype. SIZ1 physically interacts with COP1 and mediates the sumoylation of COP1. A K193R substitution in COP1 blocks its SUMO modification and reduces COP1 activity in vitro and in planta. Consistently, COP1 activity is reduced in siz1 and the level of HY5, a COP1 target protein, is increased in siz1. Sumoylated COP1 may exhibits higher transubiquitination activity than does non-sumoylated COP1, but SIZ1-mediated SUMO modification does not affect COP1 dimerization, COP1-HY5 interaction, and nuclear accumulation of COP1. Interestingly, prolonged light exposure reduces the sumoylation level of COP1, and COP1 mediates the ubiquitination and degradation of SIZ1. These regulatory mechanisms may maintain the homeostasis of COP1 activity, ensuing proper photomorphogenic development in changing light environment. Our genetic and biochemical studies identify a function for SIZ1 in photomorphogenesis and reveal a novel SUMO-regulated ubiquitin ligase, COP1, in plants.
学科主题	Genetics & Heredity
出版地	SAN FRANCISCO
WOS关键词	LIGHT-DEPENDENT INTERACTION ; BLUE-LIGHT ; CONSTITUTIVE PHOTOMORPHOGENIC1 ; ELONGATED HYPOCOTYL5 ; SEEDLING DEVELOPMENT ; GENE-EXPRESSION ; CRYPTOCHROME 1 ; PROTEIN ; SUMOYLATION ; PHYTOCHROME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000375231900046
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170170, 31471363] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2012CB114302] ; Chinese Academy of SciencesChinese Academy of Sciences [XDA08010105]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25319]
专题	中科院光生物学重点实验室
作者单位	1.King Abdullah Univ Sci & Technol, Div Biol Environm Sci & Engn, Thuwal, Saudi Arabia 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 3.Sunchon Natl Univ, Dept Biol, Sunchon, South Korea 4.Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, Japan 5.Gyeongsang Natl Univ, PMBBRC & IALS, Div Appl Life Sci BK21Plus, Jinju, South Korea 6.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lin, Xiao-Li,Niu, De,Hu, Zi-Liang,et al. An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity[J]. PLOS GENETICS,2016,12(4).
APA	Lin, Xiao-Li.,Niu, De.,Hu, Zi-Liang.,Kim, Dae Heon.,Jin, Yin Hua.,...&Jin, Jing Bo.(2016).An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity.PLOS GENETICS,12(4).
MLA	Lin, Xiao-Li,et al."An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity".PLOS GENETICS 12.4(2016).