Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1

doi:10.1107/S2053230X16013406

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	Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1
	Zhao, Shun 1; Liu, Lin
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
	2016
卷号	72 页码:795-798
关键词	ascorbic acid biosynthesis GDP-D-mannose pyrophosphorylase Arabidopsis thaliana
DOI	10.1107/S2053230X16013406
文献子类	Article
英文摘要	GDP-D-mannose pyrophosphorylase catalyzes the production of GDP-D-mannose, an intermediate product in the plant ascorbic acid (AsA) biosynthetic pathway. This enzyme is a key regulatory target in AsA biosynthesis and is encoded by VITAMIN C DEFECTIVE 1 (VTC1) in the Arabidopsis thaliana genome. Here, recombinant VTC1 was expressed, purified and crystallized. Diffraction data were obtained from VTC1 crystals grown in the absence and presence of substrate using X-rays. The ligand-free VTC1 crystal diffracted X-rays to 3.3 angstrom resolution and belonged to space group R32, with unit-cell parameters a = b = 183.6, c = 368.5 angstrom, alpha = beta = 90, gamma = 120 degrees; the crystal of VTC1 in the presence of substrate diffracted X-rays to 1.75 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 70.8, b = 83.9, c = 74.5 angstrom, alpha = gamma = 90.0, beta = 114.9 degrees.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
电子版国际标准刊号	2053-230X
出版地	CHESTER
WOS关键词	ASCORBIC-ACID SYNTHESIS ; VITAMIN-C ; BIOSYNTHESIS ; METABOLISM ; DEFICIENT ; MUTANT ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	INT UNION CRYSTALLOGRAPHY
WOS记录号	WOS:000387585000010
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170688]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25296]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Shun,Liu, Lin. Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2016,72:795-798.
APA	Zhao, Shun,&Liu, Lin.(2016).Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,72,795-798.
MLA	Zhao, Shun,et al."Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 72(2016):795-798.