CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex

doi:10.1093/pcp/pcw130

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	CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex
	Yamamoto, Hiroshi 4; Fan, Xiangyuan 3; Sugimoto, Kazuhiko ; Fukao, Yoichiro 1,2; Peng, Lianwei 3; Shikanai, Toshiharu 4
刊名	PLANT AND CELL PHYSIOLOGY
	2016
卷号	57 期号:10 页码:2122-2132
关键词	Arabidopsis Assembly factor Chloroplast Chloroplast NDH Cyclic electron transport around PSI
ISSN号	0032-0781
DOI	10.1093/pcp/pcw130
文献子类	Article
英文摘要	In vascular plants, the chloroplast NADH dehydrogenase-like (NDH) complex, a homolog of respiratory NADH: quinone oxidoreductase (Complex I), mediates plastoquinone reduction using ferredoxin as an electron donor in cyclic electron transport around PSI in the thylakoid membrane. In angiosperms, chloroplast NDH is composed of five subcomplexes and forms a supercomplex with PSI. The modular assembly of stroma-protruded subcomplex A, which corresponds to the Q module of Complex I, was recently reported. However, the factors involved in the specific assembly steps have not been completely identified. Here, we isolated an Arabidopsis mutant, chlororespiratory reduction 9 (crr9), defective in NDH activity. The CRR9 gene encodes a novel stromal protein without any known functional domains or motifs. CRR9 is highly conserved in cyanobacteria and land plants but not in green algae, which do not have chloroplast NDH. Blue native-PAGE and immunoblot analyses of thylakoid proteins indicated that formation of subcomplex A was impaired in crr9. CRR9 was specifically required for the accumulation of NdhK, a subcomplex A subunit, in NDH assembly intermediates in the stroma. Furthermore, two-dimensional clear native/SDS-PAGE analysis of the stroma fraction indicated that incorporation of NdhM into NDH assembly intermediate complex 400 was impaired in crr9. These results suggest that CRR9 is a novel factor required for the formation of NDH subcomplex A.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1471-9053
出版地	OXFORD
WOS关键词	CYCLIC ELECTRON-TRANSPORT ; DOMAIN-LIKE FOLD ; PHOTOSYSTEM-I ; NAD(P)H DEHYDROGENASE ; RESPIRATORY COMPLEX ; PHYSIOLOGICAL-ROLE ; ARABIDOPSIS-THALIANA ; FLUCTUATING LIGHT ; QUANTUM YIELD ; TOBACCO
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000388374100009
资助机构	Japan Science and Technology AgencyJapan Science & Technology Agency (JST) ; Japan Society for the Promotion of ScienceMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [25251032] ; Network of Centers of Carbon Dioxide Resource Studies in Plants ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270289]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25295]
专题	中科院光生物学重点实验室
作者单位	1.Ritsumeikan Univ, Dept Bioinformat, Kusatsu, Shiga 5258577, Japan 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020076, Japan 4.Kyoto Univ, Grad Sch Sci, Dept Bot, Kyoto 6068502, Japan 5.Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan
推荐引用方式 GB/T 7714	Yamamoto, Hiroshi,Fan, Xiangyuan,Sugimoto, Kazuhiko,et al. CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex[J]. PLANT AND CELL PHYSIOLOGY,2016,57(10):2122-2132.
APA	Yamamoto, Hiroshi,Fan, Xiangyuan,Sugimoto, Kazuhiko,Fukao, Yoichiro,Peng, Lianwei,&Shikanai, Toshiharu.(2016).CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex.PLANT AND CELL PHYSIOLOGY,57(10),2122-2132.
MLA	Yamamoto, Hiroshi,et al."CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex".PLANT AND CELL PHYSIOLOGY 57.10(2016):2122-2132.