Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex | |
Shapiguzov, Alexey1,3,4; Chai, Xin; Fucile, Geoffrey; Longoni, Paolo; Zhang, Lixin; Rochaix, Jean-David | |
刊名 | PLANT PHYSIOLOGY |
2016 | |
卷号 | 171期号:1页码:82-92 |
ISSN号 | 0032-0889 |
DOI | 10.1104/pp.15.01893 |
文献子类 | Article |
英文摘要 | Photosynthetic organisms have the ability to adapt to changes in light quality by readjusting the cross sections of the light-harvesting systems of photosystem II (PSII) and photosystem I (PSI). This process, called state transitions, maintains the redox poise of the photosynthetic electron transfer chain and ensures a high photosynthetic yield when light is limiting. It is mediated by the Stt7/STN7 protein kinase, which is activated through the cytochrome b(6)f complex upon reduction of the plastoquinone pool. Its probable major substrate, the light-harvesting complex of PSII, once phosphorylated, dissociates from PSII and docks to PSI, thereby restoring the balance of absorbed light excitation energy between the two photosystems. Although the kinase is known to be inactivated under high-light intensities, the molecular mechanisms governing its regulation remain unknown. In this study we monitored the redox state of a conserved and essential Cys pair of the Stt7/STN7 kinase and show that it forms a disulfide bridge. We could not detect any change in the redox state of these Cys during state transitions and high-light treatment. It is only after prolonged anaerobiosis that this disulfide bridge is reduced. It is likely to be mainly intramolecular, although kinase activation may involve a transient covalently linked kinase dimer with two intermolecular disulfide bonds. Using the yeast two-hybrid system, we have mapped one interaction site of the kinase on the Rieske protein of the cytochrome b(6)f complex. |
学科主题 | Plant Sciences |
电子版国际标准刊号 | 1532-2548 |
出版地 | ROCKVILLE |
WOS关键词 | PHOTOSYSTEM-II ; STATE TRANSITIONS ; PROTEIN-PHOSPHORYLATION ; ELECTRON-TRANSFER ; LIGHT ; LHCII ; PHOTOSYNTHESIS ; PLASTOQUINOL ; CHLOROPLASTS ; FLUORESCENCE |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | AMER SOC PLANT BIOLOGISTS |
WOS记录号 | WOS:000375431500006 |
资助机构 | Swiss National FoundationSwiss National Science Foundation (SNSF) [3100AO-117712] ; Foreign Expert Program of the Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/24975] |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland 2.Univ Geneva, Dept Plant Biol, Geneva, Switzerland 3.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Beijing, Peoples R China 4.Russian Acad Sci, Inst Plant Physiol, Bot Skaya St 35, Moscow 127276, Russia 5.Univ Helsinki, Dept Biosci, Div Plant Biol, Viikki Plant Sci Ctr, FI-00014 Helsinki, Finland |
推荐引用方式 GB/T 7714 | Shapiguzov, Alexey,Chai, Xin,Fucile, Geoffrey,et al. Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex[J]. PLANT PHYSIOLOGY,2016,171(1):82-92. |
APA | Shapiguzov, Alexey,Chai, Xin,Fucile, Geoffrey,Longoni, Paolo,Zhang, Lixin,&Rochaix, Jean-David.(2016).Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex.PLANT PHYSIOLOGY,171(1),82-92. |
MLA | Shapiguzov, Alexey,et al."Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex".PLANT PHYSIOLOGY 171.1(2016):82-92. |
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